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Martin, Andri Fadillah
Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology

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 1 
In vitro Growth Response of Taraxacum Officinale Weber ex F.H. Wigg Regenerated from Different Type of Explants Ermayanti, Tri Muji; Martin, Andri Fadillah
ANNALES BOGORIENSES Vol 15, No 1 (2011): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/31

Abstract

Dandelion (Taraxacum officinale Weber ex F.H. Wigg) is a medicinal plant species, reported to have some active compounds useful as anti-cancer, anti-inflammatory, antioxidants, and diuretics. Study on in vitro secondary metabolic production and tissue culture of these plants has been reported. This research was aimed to investigate plant regeneration from leaf blade, petiole and root as explants grown in selected media. Three different type of explants were cultured in MS solid medium supplemented with BAP (0, 0.5, and 1.0 mg/l) combined with NAA (0, 0.5, 1.0, and 2.0 mg/l) to select the best medium for spontaneous regeneration. Explants were grown for 6 weeks, the viability of explants, and formation of callus, shoots as well as roots were recorded. The results showed BAP at 1 mg/l combined with at 0.5 mg/l of NAA was suitable for shoot formation, whereas NAA alone was suitable for root formation from leaf blade and petiole. Root was the best explant for shoot regeneration, callus was grown at the first two weeks, and multiple shoots were grown after 3 weeks. No roots were found from root explant. The best medium for multiple shoot regeneration was MS containing 1 mg/l BAP without or with addition of 0.5 mg/l NAA.Keywords: Taraxacum officinale Weber ex F.H. Wigg, growth response, plant growth regulators, different explants, regeneration
Response of Increasing NaCl Concentrations on Growth and Proline Content of Tacca leontopetaloides cultured in vitro Martin, Andri Fadillah; Hapsari, Betalini Widhi; Ermayanti, Tri Muji
ANNALES BOGORIENSES Vol 19, No 1 (2015): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ab.v19i1.81

Abstract

Tacca leontopetaloides (L.) Kuntze which belongs to family Taccaceae is one of tuberous plant useful as an alternative food. This plant contains starch (amylose and amylopectin) similar to that of potato. In addition, this plant also contains secondary metabolites potential for diarrhea, dysentery, and anticancer. In Indonesia, this plant grows at coastal areas i.e. in Sukabumi, Yogyakarta, Garut and Karimunjawa, so this plant was suspected to have high tolerance on salinity stress. The purpose of this research was to investigate the effect of increase in NaCl concentrations on growth and its proline content of T. leontopetaloides grown in vitro. In vitro shoot tips were cultured on MS medium (Murashige & Skoog, 1962) supplemented with NaCl at concentrations of 10; 25; 50; 75; 100; 150 and 200 mM, respectively. After six weeks in culture, shoots height, shoots number, leaves number, fresh weight, as well as their proline content were recorded. The results showed that fresh weight of shoots grown on MS medium supplemented with NaCl from 10 to 100 mM was hogher compared to those of the control treatment. Fresh weight decreased when shoots were cultured on MS medium supplemented with NaCl at more than 100 mM. Height of shoots, number of shoots, and number of leaves decreased along with the increase of NaCl concentrations. The best medium for growth was MS medium supplemented with 25 mM NaCl. In this medium proline content was 15.7 umol/g FW. Proline concentrations increased along with the increase of NaCl concentrations. LD50 value was 146 mM NaCl. In conclusion that on MS medium without addition of plant growth regulators, T. leontopetaloides shoot culture was sensitve to the increase of NaCl concentration.
Micropropagation of Tetramolopium klossii (Moore) Mattfeld, endemic subalpine and alpine plant species to New Guinea Ermayanti, Tri Muji; Hafiizh, Erwin Al; Martin, Andri Fadillah; Mandessy, Ary; Hermono, Arief; Mukhsia, Andi
ANNALES BOGORIENSES Vol 15, No 2 (2011): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (415.898 KB) | DOI: 10.1234/44

Abstract

The protocol of micropropagation of Tetramolopium klossii (Moore) Mattfeld through adventitious shoot proliferation was established. This is the first report of T. klossii tissue culture. Seeds were surface-sterilized by dipping them in 10% of ethanol for 1 min followed by soaking in 1% of sodium hypochlorite for 10 min. The sterile seeds were germinated on MS medium solidified with 8 g/l of agar, supplemented with 20 g/l of sucrose. Two-month old seedlings formed some shoot clumps on MS solid medium without addition of any plant growth regulators. Small clumps containing 4-6 adventitious shoots were used for shoot proliferation on MS solid medium containing 0; 0.1; 0.5; 1.0 and 2.0 mg/L of BAP (benzylaminopurine) or kinetin. The results showed that medium MS without cytokinins (control treatment), and the addition of 0.1 or 0.5 mg/L of BAP was more effective for shoot proliferation compared to the medium with addition of BAP at higher concentration and the addition of kinetin. In further subcultures, multiple shoots grown on MS medium without cytokinins was  more vigorous  than that of shoots grown on other media, so that the shoots were ready for rooting. Medium containing 0.5 mg/L of BAP was the best for maintaining multiple shoot formation. Shoots formed roots on MS medium containing half strength of macro nutrients.   Keywords: Tetramolopium klossii, micropropagation, Murashige and Skoog medium, shoot multiplication
The Effect of Increase in NaCl Concentration on Growth and Proline Content of Purple Yam (Dioscorea alata L.) Grown In Vitro Martin, Andri Fadillah; Azizah, Farroh; Wulandari, Dyah Retno; Ermayanti, Tri Muji
ANNALES BOGORIENSES Vol 16, No 2 (2012): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/65

Abstract

Tuber of purple yam (Dioscorea alata L.) has been used as an alternative food in some areas in Indonesia. The tuber contains high carbohydrate, has low glycemic index and is gluten free. Therefore, study on genetic improvement of this species needs to be carried out in order to increase its productivity and to find out new cultivars which can be cultivated in marginal lands. This research was aimed to investigate the effect of NaCl concentration on growth and proline content of purple yam grown in vitro. Shoot tips were cultured on MS (Murashige and Skoog) medium supplemented with NaCl at concentrations of 25; 50; 100; 200 and 250 mM. After six weeks in culture, height of shoots, number of nodes, number of leaves, as well as proline content were recorded. The results showed that shoots grown on MS medium supplemented with NaCl at 25 and 50 mM had better growth compared to control. The best medium for its growth was MS containing 50 mM of NaCl. An increase in NaCl level resulted in decrease of growth. The LD50 value was obtained at 183 mM of NaCl. The highest proline concentration was achieved by shoots grown on the medium supplemented with 100 mM of NaCl. This result indicated that purple yam was tolerant to the increase of NaCl concentration up to 100 mM on MS medium without addition of plant growth regulators. Keywords: purple yam, Discorea alata L., NaCl, in vitro growth, proline content.
Co-Authors Azizah, Farroh Hafiizh, Erwin Al Hapsari, Betalini Widhi Hermono, Arief Mandessy, Ary Mukhsia, Andi Tri Muji Ermayanti Wulandari, Dyah Retno