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All Journal Jurnal Teknik Kimia USU Jurnal Teknik Kimia USU Indonesian Journal of Science and Education
Boy Fachri
Universitas Jember
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Engineering Environmental Science Industrial & Manufacturing Engineering Materials Science & Nanotechnology Physics

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The Optimization of Chlorophyll Extraction from Ulva Sp. with Ultrasound-Assisted Liquid Biphasic Systems Method Susilowati; Fakhri Arkaan Humaidi; Boy Fachri; Bekti Palupi; Meta Fitri Rizkiana; Istiqomah Rahmawati; Helda Wika Amini
Indonesian Journal of Science and Education Vol. 8 No. 1 (2024): Indonesian Journal of Science and Education
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/ijose.v8i1.1085

Abstract

Ulva sp. is a type of green algae that is easily found in the shallow seas of Indonesia and contains various bioactive compounds. Chlorophyll is a bioactive compound that functions as a natural dye, free radical scavenger, and antioxidant in the body. Chlorophyll extraction with conventional methods requires a relatively long time and large amount of solvent. In this research, chlorophyll extraction from Ulva sp. with the Ultrasound-Assisted Liquid Biphasic System (UALBS) method. Ulva sp. as much as 2.5 grams mixed in acetone solvent, added K2HPO4 solution, and carried out the sonification process in a dark room. The extraction process performs at a parameter range of 5-15 minutes, ratio 0.05-0.1 g/mL, and 60-100 mesh particle size. The extracted filtrate was added with petroleum ether and distilled water to form a biphasic condition. The extracted chlorophyll was analyzed with chromatography and spectrophotometry method. Antioxidant activity was analyzed using the DPPH method. The optimum result of Ulva sp. chlorophyll extraction at an extraction time of 10 minutes, the ratio of solids to solvent was 0.1, the particle size of 60 mesh produced a yield of 1.88% with chlorophyll a 20.13664%, chlorophyll b 21.58672% and total chlorophyll 41.71012%, and the percentage inhibition 45.32%.
Optimasi Ekstraksi Alginat dari Sargassum polycystum Menggunakan Sistem Cairan Dua Fase Berbantukan Gelombang Ultrasonik Berdasarkan Response Surface Methodology dan Pemodelan Kinetika Difusi Erica Wijaya; Effendi Utomo; Meta F Rizkiana; Yukti Nurani; Boy Fachri
Jurnal Teknik Kimia USU Vol. 15 No. 2 (2026): Jurnal Teknik Kimia USU
Publisher : Talenta Publisher (Universitas Sumatera Utara)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jtk.v15i2.26295

Abstract

Sargassum polycystum has the potential to be a source of alginate for the food and pharmaceutical industries. This study aimed to optimise alginate extraction using an ultrasound-assisted aqueous two-phase system and model the extraction kinetics based on Fick's diffusion law. Extraction was conducted using 2-propanol and K₂HPO₄, with variations in extraction time, ultrasonic power, and solid-to-solvent ratio. Optimisation was performed using response surface methodology with Box-Behnken design. FT-IR and HPLC analyses confirmed the isolation of alginate. The actual alginate yield of 81.37% was achieved at a solid-to-solvent ratio of 0.04 g/mL, an extraction time of 20 min, and an ultrasonic power of 60% (390 Watts). The optimisation results predicted an alginate yield of 82.244%, with an R² value of 0.9960 for the RSM–Box–Behnken model. The experimental yield obtained from model validation was 81.93%, with a relative prediction error of 0.38%.   The kinetic model based on Fick's diffusion law yielded a De of 1.619×10-11 and an SSE of 0.006673, indicating excellent agreement between the model predictions and the experimental data.
Optimasi Ekstraksi Alginat dari Sargassum polycystum Menggunakan Sistem Cairan Dua Fase Berbantukan Gelombang Ultrasonik Berdasarkan Response Surface Methodology dan Pemodelan Kinetika Difusi Erica Wijaya; Effendi Utomo; Meta F Rizkiana; Yukti Nurani; Boy Fachri
Jurnal Teknik Kimia USU Vol. 15 No. 2 (2026): Jurnal Teknik Kimia USU
Publisher : Talenta Publisher (Universitas Sumatera Utara)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jtk.v15i2.26295

Abstract

Sargassum polycystum has the potential to be a source of alginate for the food and pharmaceutical industries. This study aimed to optimise alginate extraction using an ultrasound-assisted aqueous two-phase system and model the extraction kinetics based on Fick's diffusion law. Extraction was conducted using 2-propanol and K₂HPO₄, with variations in extraction time, ultrasonic power, and solid-to-solvent ratio. Optimisation was performed using response surface methodology with Box-Behnken design. FT-IR and HPLC analyses confirmed the isolation of alginate. The actual alginate yield of 81.37% was achieved at a solid-to-solvent ratio of 0.04 g/mL, an extraction time of 20 min, and an ultrasonic power of 60% (390 Watts). The optimisation results predicted an alginate yield of 82.244%, with an R² value of 0.9960 for the RSM–Box–Behnken model. The experimental yield obtained from model validation was 81.93%, with a relative prediction error of 0.38%.   The kinetic model based on Fick's diffusion law yielded a De of 1.619×10-11 and an SSE of 0.006673, indicating excellent agreement between the model predictions and the experimental data.
Co-Authors Bekti Palupi Effendi Utomo Erica Wijaya Fakhri Arkaan Humaidi Helda Wika Amini Istiqomah Rahmawati Meta F Rizkiana Meta Fitri Rizkiana Susilowati Yukti Nurani