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REDUKSI KANDUNGAN AFLATOKSIN B1 (AFB1) PADA PEMBUATAN KACANG TELUR MELALUI PEREBUSAN DALAM LARUTAN KAPUR (REDUCTION OF AFLATOXIN B1 (AFB1) CONTENT IN THE EGG PEANUT BY BOILLING IN LIME SOLUTION) Yuliana Tandi Rubak; Suryana Purawisastra
Penelitian Gizi dan Makanan (The Journal of Nutrition and Food Research) Vol. 34 No. 1 (2011)
Publisher : Persagi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/pgm.v34i1.3108.

Abstract

ABSTRAK Latar belakang: Efek berbahaya dari aflatoksin B1 bagi manusia adalah toksisitasnya sebagai senyawa karsinogenik. Toksin ini diproduksi oleh Aspergillus flavus, yang biasanya tumbuh pada serealia dan kacang-kacangan. Akibatnya serealia dan kacang-kacangan tersebut serta produknya bisa terkontaminasi oleh toksin ini. Kacang telur merupakan salah satu produk kacang tanah yang banyak dikonsumsi. Karena itu, kacang telur harus bebas toksin. Menurut informasi pustaka, salah satu cara untuk menghilangkan toksin ini adalah modifikasi proses pembuatan kacang telur. Artikel ini menyajikan hasil penelitian untuk menghilangkan aflatoksin kacang telur melalui perendaman bahan baku kacang tanah dalam larutan kapur 0,5 persen dan 1 persen selama 10 menit. Metodologi: Membuat dua jenis produk kacang telur, satu produk berbahan baku kacang yang telah dikontaminasi aflatoksin dan satu produk lagi berbahan baku kacang utuh tidak terkontaminasi aflatoksin. Produk pertama untuk menguji pengaruh perebusan dalam larutan kapur terhadap kandungan aflatoksin. Sedangan produk kedua untuk menguji pengaruh perebusan dalam larutan kapur terhadap cita rasa.Kedua produk dibuat melalui proses yang sama, bahan kacang mendapat perlakukan perendaman dalam larutan kapur konsentrasi 0,5 persen dan 1,5 persen selama 10 menit, kemudian dilanjutkkan dengan proses seperti umumnya membuat kacang telur. Kandungan aflatoksin dianalisis dengan metoda ELISA (Enzyme Linked Immunosorbent Assay). ABSTRACT Background: The harmful effect of aflatoxin B1 for human being because of its toxicity, as carcinogenic agent. The toxin is produced by the Aspergillus flavus which usually grows in grain and nuts. As the result that the grain, nuts and their product contaminated by the toxin.  Egg peanut is one of peanut products which are widely consumed by the peoples. Consequently the egg peanut should be not containing the toxin. The modification of process of making peanut is one way to eliminate the toxin. Objectives: To eliminate the aflatoksin B1 content in the product of egg peanut through the preparation of raw peanuts by boiling the peanut in the boiled lime solution. The concentration of the lime solution in this research was 0.5 percent, and 1.5 percent for 10 minutes. Methods: There were two kinds of peanut egg, one was made from the raw peanuts which were already contaminated by the aflatoxin, and another one from the raw peanuts was not contaminated. The first product was to observe the influenced of boiling in the lime solution of peanuts to the content of aflatoxin. The second product was to observe the influenced of boiling in the lime solution of peanuts to the sensory of peanut eggs. Aflatoxin analysis was carried out by ELISA (Enzyme Linked Immunosorbent Assay). Results: The result of research showed that the preparation of the raw peanuts by boiling in lime solution was able to reduce the content of aflatoxin in peanut eggs. The percentage of reduction was increased by increasing the concentration of lime solution. In this study, the reduction of aflatoxin for the peanuts which was boiled in the 0.5 percent of lime solution was 27.8 percent. The reduction was increased becoming 29.5 percent when the peanuts boiled in the 1.5 percent of lime solution. The roasting of the peanut increased 44 percent the reduction of aflatoxin content. Conclusion: The modification process of making the egg peanut by boiling in lime solution could reduce the aflatoxin content, whereas the sensory of peanuts was not affected. The reduction content of aflatoxin was depending on the concentration of lime solution. [Penel Gizi Makan 2011, 34(1): 21-28] Keywords: Aflatoxin B1, peanuts, eggs peanut, lime solution, ELISA
PENYERAPAN FORMALIN OLEH BEBERAPA JENIS BAHAN MAKANAN SERTA PENGHILANGANNYA MELALUI PERENDAMAN DALAM AIR PANAS (THE ADSORPTION OF FORMALDEHYDE BY SOME FOODSTUFFS AND ITS ELIMINATION BY SOAKING THEM IN HOT WATER) Suryana Purawisastra; Emma Sahara
Penelitian Gizi dan Makanan (The Journal of Nutrition and Food Research) Vol. 34 No. 1 (2011)
Publisher : Persagi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/pgm.v34i1.3112.

Abstract

ABSTRAK Latar belakang: Penggunaan formalin sebagai bahan pengawet makanan dilarang oleh Pemerintah, karena bahan ini bersifat toksik (racun). Namun, hasil survei mengindikasikan bahwa beberapa jenis bahan makanan di pasar tradisional masih mengandung formalin. Formalin bisa digunakan sebagai bahan pengawet makanan, karena formalin dapat mengikat protein membentuk ikatan methylene (-NCHOH). Protein pada ikatan methylene ini tahan terhadap kerusakan, baik yang disebabkan oleh aktivitas mikroorganisme maupun oksidasi, sehingga makanan tersebut terhindar dari kerusakan dan menjadi awet. Artikel ini menyajikan hasil pengujian penyerapan formalin oleh beberapa jenis bahan makanan yang mengandung protein, serta pengaruh proses perendaman dalam air panas terhadap kandungan formalinnya. Metodologi: Sampel bahan makanan direndam dalam larutan formalin 1 persen. Sementara pengaruh proses perendaman dalam air panas terhadap kandungan formalin dilakukan dengan merendam bahan makanan berformalin di dalam air panas. Selama waktu periodik perendaman, bahan makanan dianalisis kadar air, formalin dan proteinnya. Hasil: Penyerapan formalin tertinggi oleh bahan makanan terjadi pada tahu, kemudian daging ayam, dan yang terendah adalah mi basah pasar. Akan tetapi, ada perbedaan tingkat penyerapan formalin antar-tahu, serta antara daging dada dan paha ayam. Kadar formalin dalam bahan makanan bisa dikurangi melalui perendaman dalam air panas. Besar pengurangan tergantung dari tingginya kandungan formalin. Sebagai contoh, kadar formalin tahu adalah 0.7 mg per g, dan menjadi 0 pada perendaman dalam air panas selama 2 jam. Kesimpulan: Penyerapan formalin oleh bahan makanan, kadarnya berbeda tergantung dari reaktivitas protein untuk bereaksi dengan formalin. Perendaman bahan makanan berformalin dalam air panas dapat mengurangi kandungannya. Rekomendasi: Perendaman bahan makanan, dengan merendam dalam air panas di rumah tangga, merupakan salah satu usaha ibu-ibu rumah tangga untuk mengurangi kadar formalin dalam bahan makanan. ABSTRACT Back ground: Due to its toxicity, the uses of formaldehyde as food preservative is prohibited by the government. However, the surveys indicated that some foodstuffs in traditional market still contained formaldehyde. The used of formaldehyde as food preservative, is due to its capability to bind the protein forming the methylene bond (-NCHOH). In this structure, the protein is resistant to the activity of spoiling the foods either to microbial or oxidation activity. This article presents the result of study in adsorption of some foodstuff containing protein to the formaldehyde, and the effect of soaking process in hot water to the reduction of formalin content in the foods. Methods: The sample of foods was soaked in 1% of formalin solution. Whereas the effect of soaking in the hot water to the formalin content of the food was conducted by soaking of the food containing formalin in hot water. During the period of soaking time then the foods were analyzed for the content of water, formaldehyde, and protein. Results: The highest adsorption of formaldehyde was in tofu, then followed by chicken and the lowest was in traditional wet-noodle. However the adsorption of formaldehyde for different sample of tofu and between chicken breast chicken legs was different. The content of formalin in the food could be reduced by soaking in hot water. The reduction was depending on the formalin content of the foods. For example, the formalin content in sample of tofu I, before soaking in the hot water was 0.7 mg per g, and becoming 0 in the 2 hours soaking time in hot water. Conclusions: The adsorption of formaldehyde in foodstuff was depending on the availability of protein to react with formaldehyde.  The soaking of the food containing formalin in hot water could be reduced its formalin content of the foods. Recommendation: The soaking of food containing formalin in hot water was an effort in households to avoid the formalin in the foods. [Penel Gizi Makan 2011, 34(1): 63-74]   Keywords: formaldehyde, adsorption, foods containing formalin, hot water