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Propagation of Sukun (Artocarpus altilis (Parkinson) Fosberg) through In Vitro Shoot Proliferation Imelda, Maria; Wulansari, Aida; Sari, Laela
ANNALES BOGORIENSES Vol 13, No 1 (2009): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/28

Abstract

Sukun (Artocarpus altilis (Parkinson) Fosberg) of the Moraceaae is a big tree which can grow to 15-20 m in height and native to  the Asia-Pasific region.  Beside its delicious fruit, sukun is also known as a  traditional herbal medicinal plant in the region, including Indonesia.  Nearly all parts of the  plant, such as roots, stems and leaves are believed by local communities to be capable of curing liver disease, hypertension, cardiac arrest, toothache, renal problem and even skin itchiness. The collaborative research between LIPI and PR China, on developing herbal medicines indicated that sukun has a great potential for treating cardiovascular disease.  However, the availability of raw materials still poses a big constraint for the industry of herbal medicines. Generally, sukun  is propagated by root or stem cuttings, since in Indonesia  sukun does not produce any seeds. However such  method only produces limited planting materials. In general tissue culture propagated plants have many advantages, namely being clonal,   free from pest and diseases, more uniform,  and  allowing a high rate of  plant multiplication. Therefore, the technique for sukun propagation has been developed by the LIPI Research Centre for Biotechnology. In this research the effects of  1-5 mg/l benzyl amino purine (BAP) and 20-40 mg/l adenine sulphate (AS) on shoot bud proliferation were investigated using lateral shoot buds on a modified Murashige and Skoog (MS) medium with addition of 150 ml/l coconut water(CW). Shoots were rooted on MS medium without plant growth regulators (PGRs). The results showed that the best medium for  in vitro shoot proliferation was a modified MS medium containing 2 mg/l BAP, 40 mg/l AS and 150 ml/l CW. The best medium for rooting is MS medium  containing 1 mg indole butyric acid (IBA), producing roots within 3 weeks. Keywords : Sukun (Artocarpus altilis),  in vitro shoot proliferation, Benzyl amino purine (BAP), Adenine sulphate (AS), coconut water (CW), Indole butyric acid (IBA)
In Vitro Propagation of Buah Merah (Pandanus Conoideus Lam) Through Lateral Bud Proliferation Imelda, Maria; Wulansari, Aida; Sumarnie, Sumarnie
ANNALES BOGORIENSES Vol 12, No 1 (2008): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2552.626 KB) | DOI: 10.1234/61

Abstract

Pandanus  conoideus Lam  or  Buah  merah  of the  Pandanaceae  is  native  to  East  Indonesia,  particularly Papua and  North Maluku. Traditionally  the  fruits  are  used  for health  promotion and maintenance as well  as  for curing  several  illnesses.  Recently,  it  has  been  reported  that  the  fruits  are  potential  for  cancer medication . As a result, there  has  been  an  overexploitation of the  plants  from  their habitats .  In  order  to  anticipate  their possible disappearance due to overexploitation in the wild, an efficient and effective technology for the mass propagation, conservation and  cultivation  of these plants should be developed. Generally  buah merah is propagated vegetatively by  offshoots and stem cuttings or generatively by seeds.  Micropropagation  has  many  advantages  over  the conventional method. because  the  technique allows mass  cIonal  and  pathogen-free  production of plants  at high  rate of multiplication all  year round . In  this  research the  effects of 0,1 -0.2 mg/l thidiazuron  (TDZ), 0.5- 1.0 benzyl  amino  purine  (BAP)  and  0.25-0.5  mg/l  Kinetin  (KN)  on  shoot  bud  induction  and  proliferation of P.  conoideus were  investigated  using  nodal   sections  or  lateral buds  or P.  conoideus  on modified  Murashige and skoog medium. Shoots were rooted on MS medium  without plant growth  regulators (PGR) . The  result  showed that  lateral  buds  of Pandanus  started  to  initiate  growth after 4-7  days  in  culture.  The  best medium  for  shoot proliferation  was MS  containing either 0,5 mg/l  BAP with  0.1 mg/ l  TDZ or  I  mg/l  BAP with  0.5  mg/I  KN. giving a multiplication rate of  I6.5  shootlets per shootbud explant after 8 weeks Rooting of shoots was successfully conducted on MS medium without PGR.  Acclimatization of  rooted plantlets was achieved on  a mixed medium of  cocopeat and soil (1:1). Keywords: Buah merah (Pandanus conoideus). lateral buds, BAP. TDZ. KN