I Gede Swibawa
Jurusan Proteksi Tanaman, Fakultas pertanian, Universitas Lampung, Bandar Lampung

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Uji Efektivitas Trichoderma asperellum dalam Pengendalian Nematoda Puru Akar (Meloidogyne spp.) Secara In Vitro Sisi Indriyani; I Gede Swibawa; Ni Kadek Emi Sintha Dewi; Cipta Ginting
Jurnal Proteksi Agrikultura Vol. 3 No. 1 (2026): MAY, JURNAL PROTEKSI AGRIKULTURA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpa.1315-20

Abstract

Root-knot nematodes (Meloidogyne spp.) are significant pests that damage the root systems of plants and are difficult to control using conventional methods. The use of chemical nematicides carries the risk of environmental contamination, so environmentally friendly control alternatives are needed, one of which is using the biological agent Trichoderma asperellum. This study aims to determine the effect of spore concentration and exposure duration of T. asperellum on the hatching of Meloidogyne spp. eggs in vitro. The research was conducted at the Plant Pest Science Laboratory and the Biotechnology Laboratory of the Faculty of Agriculture, University of Lampung, from May to July 2025. The experiment used a completely randomized design (CRD). The first experiment tested five concentrations of spore suspension dilutions (10-1, 10-2, 10-3, 10-5, and a control), while the second experiment tested exposure durations of 10, 15, 30, and 60 minutes. The results showed that a concentration of 10-2 (2.7 × 105 spores/mL) was able to inhibit egg hatching by 50.90%, which was higher than the other concentrations. In the duration test, juvenile mortality increased over time, reaching 85.37% at 10 minutes and 99.83% at 60 minutes. In conclusion, a 10-2 concentration with a minimum exposure of 30–60 minutes is effective in reducing the hatching rate of Meloidogyne spp. eggs and has the potential to be applied as an environmentally friendly biological control.
Pengaruh implementasi Netamax-FP Unila terhadap keragaman dan kelimpahan nematoda dalam tanah pada pertanaman guava kristal di Lampung Aulia Shalsha Saharani; I Gede Swibawa; Selvi Helina; Radix Suharjo
Jurnal Proteksi Agrikultura Vol. 3 No. 1 (2026): MAY, JURNAL PROTEKSI AGRIKULTURA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpa.1321-30

Abstract

Crystal guava (Psidium guajava L.) is a horticultural plant whose fruit is widely known and as a favorable fruit in Indonesia. Crystal guava productivity in Lampung is relatively lower compared to that in Central Java and East Java. One of the factors causing a decline in crystal guava production is pest attack, including plant-parasitic nematodes. The aim of this study was to determine the effect of Netamax-FP Unila bionematicide application on the composition of the nematode community and its effects on the diversity and abundance of nematodes in crystal guava plantations at PT Great Giant Food. The research was conducted from September 2023 to March 2024. Nematode observations were carried out before and after application bionematicide. Nematodes were identified to the genera taxonomic level. Nematode diversity was measured using the number of genera, funtional groups, the Shannon-Wiener index, species richness index, species evenness index and Simpson's index. Nematode abundance was measured base on the number of individuals of each genus. The research results showed that 16nematode genera were identified. These genera were classified into three functional groups, including parasitic nematodes (herbivores), bacterial-feeding nematodes (bacterivores), and fungal-feeding nematodes (fungivores). The application of the Netamax-FP Unila bionematicide combined with compost reduced the abundance of plant-parasitic nematodes without having a negative impact on free-living nematodes or overall nematode diversity.