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All Journal International Journal of Oil Palm
Delia Agustina
PT Wilmar Benih Indonesia, Cikarang 17530, Indonesia
Agriculture, Biological Sciences & Forestry Decision Sciences, Operations Research & Management Environmental Science

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A Rapid Inoculation Method for Infection of Ganoderma in Oil Palm Maria Indah Purnamasari; Delia Agustina; Cahya Prihatna; Antonius Suwanto
International Journal of Oil Palm Vol. 1 No. 1 (2018): January 2018
Publisher : Indonesian Oil Palm Society /IOPS (Masyarakat Perkelapa-sawitan Indonesia /MAKSI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35876/ijop.v1i1.1

Abstract

Basal stem rot (BSR) is a major disease of oil palm plantation caused by pathogenic fungus Ganoderma boninense. To overcome the disease, development of resistant or tolerantoil palms is crucial for susta inable production of palm oil. Thus a reliable and rapid method to assess resistance of oil palm to BSR is important. Here we report an inoculation technique designated as mycelium inoculation technique. This method is faster and simpler compared to the traditional technique using rubber wood block. The technique that we developed results in consistency of infection and disease can be evaluated as early as two weeks after inoculation with G. boninense. Furthermore, this method is also able to di?erentially assess resistant and susceptible palm seedlings to BSR. The results show that this new inoculation technique can be used as a routine method to infect oil palm seedlings and can be utilised for development of resistant cultivar of oil palm to G. boninense.
Rapid Inoculation Technique and Biological Control of Leaf Spot Disease in Oil Palm Delia Agustina; Cahya Prihatna; Antonius Suwanto
International Journal of Oil Palm Vol. 2 No. 1 (2019): January 2019
Publisher : Indonesian Oil Palm Society /IOPS (Masyarakat Perkelapa-sawitan Indonesia /MAKSI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35876/ijop.v2i1.24

Abstract

Oil palm (Elaeis guineensis) is one of the most efficient oil-producing crops in the world. However, fungal diseases such as basal stem rot (BSR) caused by Ganoderma sp. causes significant loss to the yield of adult palms. Apart from BSR, leaf spot disease caused by Curvularia sp. also causes significant loss during nursery stages. In this study, Curvularia sp. was isolated from a diseased palm seedling and a rapid and reproducible artificial inoculation method was developed. The technique has bioassay to determine the level of success of the control of leaf spot disease in a glasshouse setting. A natural, organic cyclic peptide fungicide and living cells of a bacterial strain Paraburkholderia sp. CP01 were tested for their efficacy to control leaf spot in oil palm seedlings. The severity of leaf spot disease in oil palm seedlings treated by organic cyclic peptide fungicide and CP01 was significantly lower than untreated control, indicating potential biological control agents. The results presented here provide technical reference and novel approach to controlling leaf spot disease of oil palm.
Co-Authors Antonius Suwanto Cahya Prihatna Maria Indah Purnamasari