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Riana Putri Rahmawati
Universitas Muhammadiyah Kudus, Indonesia
Health Professions

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Formulation and Efficacy Test of Antistress Aromatherapy Candle with Jasmine Essential Oil (Jasminum sambac L) on Mice (Mus musculus) Noor Alfina Febrianti; Riana Putri Rahmawati; Fahrudin Arif
Advances in Healthcare Research Vol. 3 No. 2 (2025)
Publisher : Yayasan Pendidikan Bukhari Dwi Muslim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.60079/ahr.v3i2.521

Abstract

Purpose: Aromatherapy is a non-pharmacological healing method that uses essential oils as therapeutic agents. Jasmine flowers (Jasminum sambac L.) contain active compounds such as linalool, benzyl acetate, methyl salicylate, cis-jasmone, nerolidol, and indole that have potential applications in stress management. This study aims to investigate the antistress efficacy of aromatherapy candles made from jasmine essential oil on mice (Mus musculus). Research Method: This study employed a laboratory experiment with a post-test-only control group design. A total of 25 male mice were divided into five groups, each consisting of five mice. The treatment groups were exposed to aromatherapy candles with essential oil concentrations of 2%, 4%, and 6%, while the control groups included a negative control and a positive control. Stress induction was performed using the Tail Suspension Test (TST), and effectiveness was assessed through immobility time parameters. Data analysis was conducted using a One-Way ANOVA test. Results and Discussion: The results indicate that the wax formula with a concentration of 6% has the best physical characteristics and provides the fastest relaxation effect. The ANOVA test reveals a significant difference between groups (p < 0.05), indicating a significant effect of aromatherapy wax on stress reduction in mice. Implications: This study reinforces the potential of jasmine essential oil-based aromatherapy candles as an alternative therapy for stress management, while also supporting the development of safe and effective, naturally based health products.
Formulation and Efficacy Test of Antistress Aromatherapy Candles Containing Peppermint Leaf Essential Oil (Mentha Piperita L) and Clove Leaf Essential Oil (Syzygium Aromaticum L) on Mice (Mus Musculus) Khunut Nazilla; Riana Putri Rahmawati; Bintari Tri Sukoharjanti
Advances in Healthcare Research Vol. 3 No. 2 (2025)
Publisher : Yayasan Pendidikan Bukhari Dwi Muslim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.60079/ahr.v3i2.523

Abstract

Purpose: This study aims to determine the effectiveness of aromatherapy candles containing peppermint (Mentha piperita L.) and clove (Syzygium aromaticum L.) essential oils at concentrations of 3%, 5%, and 7% on mice. Research Method: This study employed a laboratory experimental method with a post-test-only control group design. The formula used in this study was a combination of peppermint and clove with concentrations FII (2%:1%), FIII (3%:2%), and FIV (4%:3%), as well as negative and positive controls (lavender). The evaluation was conducted on physical tests of the candles, including organoleptic tests, burn time tests, and melting point tests. The antistress effectiveness testing was divided into five groups, each consisting of 5 mice. The first treatment involved the Tail Suspension Test to induce stress in the mice. Subsequently, the mice were exposed to aromatherapy candles, and the Forced Swimming Test was conducted. The test results were analyzed using the One-Way ANOVA test. Results and Discussion: All aromatherapy candle formulas met the physical evaluation test requirements. Formulas FII and FIII demonstrated antistress efficacy with a significance value of less than 0.05. Implications: These results indicate that the combination of peppermint leaf essential oil and clove leaf essential oil has potential as an active ingredient in anti-stress aromatherapy candles. Further research is needed to support its effectiveness on a broader scale.
Testing the Effectiveness of Basil Leaf Essential Oil (Ocimum basilicum L) against Aedes Aegypti Mosquitoes in Aromatherapy Candles Santi Dwi Rahayu; Riana Putri Rahmawati; Fahrudin Arif
Advances in Healthcare Research Vol. 3 No. 2 (2025)
Publisher : Yayasan Pendidikan Bukhari Dwi Muslim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.60079/ahr.v3i2.541

Abstract

Purpose: This study aims to formulate basil leaf essential oil (Ocimum basilicum L.) as an active ingredient against Aedes aegypti mosquitoes in the form of aromatherapy wax that can be applied by inhalation. Research Method: This study is a laboratory experimental study. Essential oil from basil leaves was obtained through steam distillation, while the wax preparation was made using the melting method. The aromatherapy wax formulation consisted of five groups, namely positive control, negative control, formulation I (2%), formulation II (4%), and formulation III (6%). Each formulation was tested through a series of physical property tests, including organoleptic tests (color, odor, and shape), burning time test, melting point test, and effectiveness test as an insect repellent against Aedes aegypti. Data analysis was performed using a one-way ANOVA statistical test and followed by a Tukey Post Hoc test. Results and Discussion: The results showed that aromatherapy candles containing basil essential oil had varying effectiveness against mosquito mortality. Formulations with higher concentrations (4% and 6%) provided the best results in physical testing and biological effectiveness compared to other formulations and controls. Implications: This study supports the development of natural-based aromatherapy candles as a safe, effective, and environmentally friendly alternative insecticide for controlling Aedes aegypti mosquitoes in domestic environments.
Co-Authors Bintari Tri Sukoharjanti Fahrudin Arif Khunut Nazilla Noor Alfina Febrianti Santi Dwi Rahayu