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All Journal Buletin Keslingmas Proceeding Mulawarman International Conference on Tropical Public Health
Riyan Ningsih
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Environmental Science Health Professions Public Health

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Identifikasi Kandungan Bakteri Coliform Pada Air Es Batu Di Rumah Makan Sukma, Anggun Mutiara; Riyan Ningsih; Vivi Filia Elvira; Syamsir; Ayudhia Rachmawati
Buletin Keslingmas Vol. 44 No. 4 (2025): BULETIN KESLINGMAS: VOL. 44 NO. 4 TAHUN 2025
Publisher : Poltekkes Kemenkes Semarang

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Coliform bacteria are an indicator of contamination in foodstuffs that can cause waterborne diseases such as diarrhea. Ice cubes are homemade food products produced only from water in plastic bags and then frozen. The increased risk of ice cube contamination can occur from the manufacturing process to serving buyers. This study aims to identify the presence of Coliform bacteria in ice cubes sold in restaurants in Jalan Pramuka, Samarinda City. This study used descriptive quantitative methods and the Most Probable Number (MPN) method for the analysis of ice cube water samples. Samples were taken from 22 restaurants and analyzed at the East Kalimantan Regional Health UPTD laboratory. The results showed that 86.4% of restaurants did not meet sanitary hygiene standards, and more than 50% of ice cube samples tested had Coliform bacteria counts exceeding the threshold set by Permenkes No. 2 Year 2023. These findings indicate the potential for serious health risks to consumers. Conclusions and suggestions from this study should improve sanitation supervision in the ice cube production process as well as education to sellers regarding the importance of hygiene in maintaining food quality. This study is expected to serve as a reference for authorities in sanitation policy making and increase public awareness of health risks due to Coliform bacteria contamination.
Food Hygiene and Sanitation at Beluluq Lingau Thematic Market(Dayak Market), Samarinda City, 2025 Karolina Puspitasari; Riyan Ningsih; Blego Sedionoto
Mulawarman International Conference on Tropical Public Health Vol. 2 No. 2 (2025): The 4th MICTOPH
Publisher : Faculty of Public Health Mulawarman University, Indonesia

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Background : The hygiene and sanitation conditions of traditional markets are essential for ensuring community food safety. The Beluluq Lingau Thematic Market in Samarinda City promotes local Dayak culture and offers both general and traditional East Kalimantan foods Objective : This study aimed to describe food hygiene and sanitation conditions at Beluluq Lingau Market and to assess microbiological quality based on the coliform parameter Research Methods/ Implementation Methods : This research used an analytical descriptive design with a cross-sectional approach. Samples were total sampling from 6 food stalls and 10 food handlers. Data were collected through observation and interviews using inspection form from the Indonesian Ministry of Health (Peraturan Pemerintah No. 66/2014). Microbiological analysis was conducted on seven food samples using secondary data from Puskesmas Sempaja from routine monitoring with sanitarian kit. The parameters tested included total coliform and Escherichia coli. Results : Result showed that 50% of food stalls did not meet hygiene and sanitation requirements. The unmet aspects included covered waste bins (100%), use of masks and aprons (100%), and vector control (100%). A total of five out of seven food samples (71.4%) tested positive for coliform, with bacterial counts exceeding 100, two negative samples were traditional snacks wrapped in plastic and leaves. Conclusion/Lesson Learned : Food stalls at the Beluluq Lingau Market still require improvement in hygiene and sanitation to prevent microbiological contamination. Strengthening food safety training and ensuring the availability of sanitation facilities such as food covers during serving, personal protective equipment, and covered waste bins are essential
ANTIMICROBIAL RESISTANCE PATTERN OF CLINICAL BACTERIAL ISOLATES AT THE EAST KALIMANTAN PROVINCIAL HEALTH LABORATORY, 2024 Rahmadi; Riyan Ningsih; Blego Sedionoto
Mulawarman International Conference on Tropical Public Health Vol. 2 No. 2 (2025): The 4th MICTOPH
Publisher : Faculty of Public Health Mulawarman University, Indonesia

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The rising incidence of infections caused by antimicrobial-resistant bacteria has become a serious public health concern in Indonesia, yet data on resistance patterns in East Kalimantan, particularly in Samarinda, remain limited. This study aimed to describe antimicrobial resistance patterns among clinical bacterial isolates examined at the East Kalimantan Provincial Health Laboratory (UPTD Labkes) in 2024 through a descriptive observational design using secondary data from culture results and antibiotic susceptibility testing of 201 clinical isolates collected from January to December 2024. Data were analyzed descriptively using tabulation and percentage distribution. Of all positive isolates, 54.4% were Gram-negative bacteria and 45.6% were Gram-positive, with Escherichia coli being the most frequently identified species (24.4%), followed by Staphylococcus haemolyticus (7.0%) and Pseudomonas aeruginosa (7.0%). Susceptibility testing indicated that E. coli exhibited high sensitivity to meropenem (98%) and amikacin (100%), whereas Staphylococcus aureus demonstrated oxacillin resistance of 30.8%, suggesting the presence of MRSA. The predominant resistance mechanisms detected included β-lactamase production (21.7%), ESBL (40.6%), and MRSA (37.7%). These findings highlight substantial variability in bacterial susceptibility patterns in Samarinda and provide an essential baseline for strengthening continuous antimicrobial resistance surveillance efforts.
Co-Authors Ayudhia Rachmawati Blego Sedionoto Elvira, Vivi Filia Karolina Puspitasari Rahmadi Sukma, Anggun Mutiara Syamsir