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Menik Kasiyati
Department of Medical Laboratory Technology, Poltekkes Kemenkes Yogyakarta, Yogyakarta, Indonesia
Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Health Professions Immunology & microbiology Public Health

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Air Conditioner Condensate and Mineral Water as Alternative Solvents for Potato Dextrose Agar: Comparative Growth of Trichophyton rubrum Martha Atik Martsiningsih; Yusmiarti Yusmiarti; Anik Nuryati; Siti Nur Azizah; Menik Kasiyati; Budi Setiawan
JURNAL INFO KESEHATAN Vol 24 No 1 (2026): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol24.Iss1.2239

Abstract

Trichophyton rubrum is the predominant cause of dermatophytosis worldwide, particularly in tropical countries such as Indonesia. Potato Dextrose Agar (PDA) is a standard medium for fungal culture, conventionally prepared with distilled water; however, its limited availability and high cost in resource-constrained laboratories necessitate alternative solvents. This study aimed to evaluate the growth of T. rubrum on PDA prepared with distilled water, air conditioner (AC) condensate, and bottled mineral water by comparing colony diameter and morphology. A quasi-experimental study with a post-test-only non-equivalent control group design was conducted. PDA was prepared using the three solvents, inoculated by the single-dot method, and incubated at a fixed temperature of 28°C for 7 days. Colony diameter and macroscopic and microscopic morphology were assessed. Statistical analyses included the Shapiro–Wilk test, One-Way ANOVA, and Independent Sample T-test (p < 0.05). All media supported typical T. rubrum growth. Colony diameters obtained using AC condensate were not significantly different from those obtained using distilled water, whereas mineral water resulted in significantly smaller colonies. These findings indicate that AC condensate can serve as an effective and economical substitute for distilled water in PDA preparation, while the use of mineral water should be limited due to variability in composition and lack of standardization.      
Analysis of NLR and MLR Values in Patients with Pulmonary Tuberculosis and Pulmonary Tuberculosis with Diabetes Mellitus Rachmad Bayu Kuncara; Parisade Galih Sasangka; Anung Sugihantono; Iin Desmiany Duri; Nurul Qomariyah; Menik Kasiyati; Sresta Azahra; Wiwit Sulistyasmi
Jurnal Kesehatan Vol 16 No 2 (2025): Jurnal Kesehatan
Publisher : Poltekkes Kemenkes Tanjung Karang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jk.v16i2.5149

Abstract

Tuberculosis (TB) infection can cause changes in hematological parameters such as neutrophils, monocytes, and lymphocytes. The neutrophil-to-lymphocyte ratio (NLR) and monocyte-to-lymphocyte ratio (MLR) are used as indicators of inflammation and immune response in infectious diseases, including TB. Diabetes mellitus (DM) as a comorbidity can exacerbate inflammation, potentially affecting NLR and MLR values in TB patients. This study aimed to determine the NLR and MLR values in patients with pulmonary TB and pulmonary TB with DM. This research used a quantitative observational design with a cross-sectional approach, involving 72 respondents from several primary health centers (Puskesmas) in Semarang City, divided into two groups of 36 individuals each. The sampling technique used was purposive sampling. The results showed that the mean NLR value in pulmonary TB patients was 2.20, and in pulmonary TB with DM patients was 2.89, with a significant difference (p=0.002). Meanwhile, the mean MLR value in pulmonary TB patients was 0.34 and in pulmonary TB with DM patients was 0.33, with no significant difference (p=0.752). These findings indicate that NLR is more sensitive than MLR in reflecting the inflammatory status and DM complications in TB patients. NLR can be used as an additional marker in the clinical monitoring of TB patients, especially those with DM comorbidity.
Co-Authors Anik Nuryati Anung Sugihantono Budi Setiawan Iin Desmiany Duri Martha Atik Martsiningsih Nurul Qomariyah Parisade Galih Sasangka Rachmad Bayu Kuncara Siti Nur Azizah Sresta Azahra Wiwit Sulistyasmi Yusmiarti Yusmiarti