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All Journal Journal of Dentistry Indonesia
Ramasamy, Pasiyappazham
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Dentistry

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Synthesis and Partial Characterization of Chitosan Phosphorylation from Marine Cuttlefish Sepia aculeata (Orbingy, 1848) and Its Antimicrobial Potentials Against Oral Pathogens Putta, Yathin Reddy; Pitchai, Annathai; Ramasamy, Pasiyappazham
Journal of Dentistry Indonesia Vol. 32, No. 1
Publisher : UI Scholars Hub

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Abstract

Objectives: This study investigated the synthesis and partial characterization of phosphorylated chitosan extracted from the cuttlebone of Sepia aculeata (Orbingy, 1848) and evaluated its antimicrobial potential against oral pathogens. Methods: The synthesis of phosphorylated chitosan from Sepia aculeata cuttlebone involved several steps: cleaning, deproteinization, demineralization to extract chitin, deacetylation to produce chitosan, and phosphorylation to introduce phosphate groups. Phosphorylation enhances the properties of chitosan, making it a promising candidate for oral health applications. Phosphorylated chitosan was purified and characterized using Fourier Transform Infrared Spectroscopy (FTIR), X-ray diffraction (XRD), and Scanning Electron Microscopy (SEM) to confirm structural changes. This process enhances the properties of chitosan and promotes its sustainability by utilizing marine waste materials. Antimicrobial activity was assessed using the well diffusion method. Results: Results showed maximum inhibition against Pseudomonas aeruginosa (27 ± 2.25 mm) and minimum inhibition against Candida tropicalis (23 ± 1.83 mm), as confirmed by FT-IR spectroscopy, SEM, and XRD. Conclusion: Antimicrobial assays showed significant efficacy against common oral pathogens, indicating their potential use in dental-care products.
Co-Authors Pitchai, Annathai Putta, Yathin Reddy