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Identifikasi Fraksi Bioaktif Bawang Merah Palu (Allium x wakegi Araki.) sebagai Antibakteri terhadap Salmonella typhi dan MRSA melalui KLT-Bioautografi Sari, Sitti; Fahru, Almira Rayyah Shadriah
Jurnal Biologi Babasal Vol. 4 No. 1: Edisi April 2025
Publisher : Program Studi Pendidikan Biologi FKIP Uniiversitas Muhammadiyah Luwuk

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32529/jbb.v4i01.3967

Abstract

Bacterial resistance to antibiotics encourages the development of alternative antibacterial agents based on natural ingredients. Shallots (Allium x wakegi Araki.) Palu cultivars are known to contain secondary metabolites such as flavonoids, alkaloids, and steroids that have the potential as antibacterials. This study aims to evaluate the antibacterial activity of the ethanol extract fraction of Palu shallot bulbs against Salmonella typhi and Methicillin-Resistant Staphylococcus aureus (MRSA), and to identify the bioactive compounds contained therein. The thick extract was obtained through 96% ethanol maceration and then fractionated using Vacuum Liquid Chromatography (VLC). Identification of compounds was carried out using Thin Layer Chromatography (TLC) and specific detection reagents (Dragendorff, Liebermann-Burchard, AlCl?, FeCl?). The antibacterial activity of each fraction was tested using the direct bioautography method. The results of the study are expected to reveal the active fractions and the dominant bioactive compounds as natural antibacterial candidates. These findings are expected to support the utilization of local biological resources as an alternative in the development of plant-based antimicrobial agents, especially from the Palu red onion cultivar which has high adaptive and bioactive potential.
Research-Based Teaching E-Modules on Mangrove Diversity to Enhance Meaningful Learning in Banggai Regency Haruna, Moh. Fahri; Fahru, Almira Rayyah Shadriah; S, Siti Marwah; Katili, Abubakar Sidik; Odja, Abdul Haris
Jurnal Penelitian Pendidikan IPA Vol 11 No 11 (2025): November
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i11.13084

Abstract

The importance of mangrove ecosystems such as shoreline buffers, biodiversity habitats, and carbon sinks is increasingly threatened by anthropogenic degradation. This study aimed to develop and validate a meaningful learning–based e-module relevant to the context of mangrove diversity in Banggai Regency. Mangrove diversity was utilized as teaching material in the developed e-learning module to provide students with early knowledge about mangroves, enabling them to protect and conserve mangrove ecosystems in their environment. The method employed was a three-stage Research and Development (R&D) approach: field exploration, e-module development based on field data and meaningful learning principles, and product validation by subject matter experts, media experts, and practitioners through observations and questionnaires. Data were analyzed using descriptive qualitative and quantitative methods. The results showed that this interactive e-module, through a limited trial involving 30 students, achieved an average feasibility score of 89.7%. The discussion confirmed that the application of meaningful learning principles integrated with local contexts proved highly valid and effective in improving students' understanding of mangrove diversity. In conclusion, this e-module is feasible and can be implemented to support conservation efforts and enhance students’ understanding. (The total word count is approximately 145, which is concise and covers all essential elements).