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All Journal Jurnal Biodjati Makara Journal of Science
Adawiah, Adilah
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Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences

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Isolation and Selection of Bacillus spp. as Candidate Probiotics for Use in Chickens Adawiah, Adilah; Wiryawan, Komang Gede; Meryandini, Anja
Makara Journal of Science
Publisher : UI Scholars Hub

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Abstract

The growth rate of chickens can be improved by using probiotics as alternatives to antibiotics. Bacillus spp. as probiotics are particularly beneficial due to ability to form spores, which remain stable as commercial products over extended periods. This study aimed to isolate and select Bacillus spp. from Jasinga soil as probiotic candidates for chickens. A total of 15 isolates obtained of these, 12 were identified as Bacillus spp based on microscopic observations and screening test including ascertaining hemolysis, protease, amylase, and cellulase activities. Only six isolates exhibited gamma hemolysis and the ability to hydrolyze proteins, starch, and cellulose. Isolates BTJ1.1, 1.2, 1.4, and 2.3 poliferated in 0.3% bile salts. BTJ1.1, 1.2 and 2.3 also exhibited antimicrobial activity and sensitivity to amoxicillin, tetracycline, and rifampicin. BTJ1.1 and 2.3 were highly hydrophobic, a key probiotic trait. 16S rRNA gene sequence analysis revealed that BTJ1.1 and 2.3 were >99% similar to B. amyloliquefaciens strain B54 and B. subtilis strain ASI3, respectively, highlighting their potential as probiotics.
Exploration and Identification of Ectomycorrhizal Fungi at IPB University Campus Forest Adawiah, Adilah; Mufidah, Dinda Rista; Putra, Ivan Permana
Jurnal Biodjati Vol 8 No 2 (2023): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v8i2.26109

Abstract

The exploration of ectomycorrhizal fungi diversity around the campus could provide insights into the presence and distribution of these symbiotic fungi in the area. This study aimed to observe, identify, and provide information regarding ectomycorrhizal fungi diversity in the IPB University Campus Forest. The sampling sites represent various tree species including forests, parks, and green spaces. The exploration was done using opportunistic sampling method. The obtained basidiomata were examined for macromorphological and micromorphological examination to identify the ectomycorrhizal fungi. The identification results based on the morphological characteristics confirmed our specimens as Inocybe cf. squarrosolutea (Corner & E. Horak) Garrido and Suillus bovinus (L.) Roussel. The Inocybe was characterized by having small to medium-sized, pileus squamules, dry surface, light brown to yellowish cap, lamellae crowded, cylindrical or attenuated stipe towards the apex, clavate basidia, and hemispheric knobs basidiospores. The Suillus was distinguished by having a cap that is convex in shape with a slightly brownish yellow color and the surface texture of the cap is smooth, lamella in the form of pores that are quite large and angular, brownish yellow in color, yellow tubular stipe, clavate basidia, and oval spores. These two macrofungi are new records in the sampling area and I. cf. squarrosolutea could be the new information for Indonesia. Our finding provides valuable information for ecological studies and contributes to the additional data on Indonesian ectomycorrhizal fungi diversity.
Co-Authors Anja Meryandini I Komang Gede Wiryawan Ivan Permana Putra Mufidah, Dinda Rista