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Goenharto, Setyabudi
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Dentistry

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Antibiofilm activity of probiotic bacteria Lactobacillus plantarum FNCC 0020 against Streptococcus mutans serotype c Pribadi, Nirawati; Sampoerno, Galih; Juniarti, Devi Eka; Goenharto, Setyabudi; Zubaidah, Nanik; Ramadhini Arrianti, Tyas; Ramadhani, Aqila Shabrina Dwi; Fadhillah, Revita Rizki
Conservative Dentistry Journal Vol. 15 No. 1 (2025): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v15i1.2025.33-37

Abstract

Background: Streptococcus mutans (S. mutans) is a Gram-positive bacterium that plays a role in dental caries. Plaque and biofilm formation can be chemically cleaned using mouthwash, such as chlorhexidine gluconates (CHX) 0.2%, which is the gold standard. Another alternative can be developed to inhibit S. mutans biofilm formation by using Lactobacillus plantarum probiotic, which has antibiofilm potency by producing antimicrobial substances. Purpose: This study tested the biofilm inhibition to determine the Inhibitory Concentration that can inhibit 50% of biofilm formation. Methods: This research was conducted as an in vitro experimental laboratory study. Biofilm inhibition testing was performed by using the microdilution method on biofilms formed in 96 well microplates with 0,1% crystal violet staining. Lactobacillus plantarum FNCC 0020 was diluted to several concentrations: 12.5%, 25%, and 50% against Streptococcus mutans biofilm induced by 3% sucrose. The test results in optical density were read using a spectrophotometry with a wavelength of 650 nm. were determined using the GraphPad Prism sigmoidal dose-response method. Results: There is antibiofilm activity of Lactobacillus plantarum FNCC 0020 against Streptococcus mutans, and the inhibitory effect against Streptococcus mutans biofilm showed of 42.43%. Conclusion: Lactobacillus plantarum FNCC 0020 at 50% has biofilm inhibitory activity compared to other concentrations but is less effective than 0.2% CHX.
Effect of nano calcium carbonate from Anadara granosa shell induced odontoblast-like cells as a pulp capping material candidate Saraswati, Widya; Ismiyatin, Kun; Goenharto, Setyabudi; Kunarti, Sri; Zubaidah, Nanik; Halima, Siti Noorraida; Daradjat, Kinanti Naianuha; Gusniarti, Haninda Dhipta
Conservative Dentistry Journal Vol. 15 No. 1 (2025): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v15i1.2025.43-45

Abstract

Background: Vital pulp therapy is a treatment to repair and maintain pulp vitality. Calcium hydroxide is the gold standard in pulp capping materials. Calcium hydroxide has advantage antibacterial. However, calcium hydroxide has some disadvantages such form a layer liquefaction necrosis. Blood clam shells contain 98.7% calcium carbonate. Calcium carbonate (CaCO3) content becomes a good source of calcium can be used as a bone repair material. Purpose: To review effect nano CaCO3 from Anadara granosa shell induced odontoblast-like cells as a pulp capping candidate. Nanoparticles CaCO3 has advantages such as high surface area, low toxicity, biocompatibility, biodegradability, pH-sensitivity, and osteoconductivity. Reviews: Nano CaCO3 from Anadara granosa are promising to promote odontoblast-like cells formation. Conclusion: Nano CaCO3 its potential to influence odontoblast-like cells, which play a crucial role in dentin regeneration and repair.
Co-Authors Daradjat, Kinanti Naianuha Devi Eka Juniarti Fadhillah, Revita Rizki Galih Sampoerno Gusniarti, Haninda Dhipta Halima, Siti Noorraida Kun Ismiyatin Nanik Zubaidah Nirawati Pribadi Ramadhani, Aqila Shabrina Dwi Ramadhini Arrianti, Tyas Sri Kunarti, Sri Widya Saraswati