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All Journal Chimica et Natura Acta JURNAL CRANKSHAFT Jurnal Teknologi
Rinaldi, Kardian
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Electrical & Electronics Engineering Energy Engineering Environmental Science Materials Science & Nanotechnology Mechanical Engineering

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Penentuan Kurva Pertumbuhan Lactococcus lactis dan Evaluasi Potensi Supernatan Bebas Sel sebagai Agen Antibakteri terhadap Escherichia coli Krista, Gustin Mustika; Rinaldi, Kardian; Rahma, Andien Tiara; Maswanti, Azzahra Junisa
Jurnal Teknologi Vol 25, No 2 (2025): Agustus 2025
Publisher : Politeknik Negeri Lhokseumawe

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30811/teknologi.v25i2.7504

Abstract

The increasing resistance of pathogenic bacteria to synthetic antibiotics has driven the search for safer and more sustainable natural antimicrobial agents. Lactic acid bacteria, particularly Lactococcus lactis, are known to produce bioactive compounds such as bacteriocins that can inhibit the growth of pathogenic microorganisms. This study aimed to evaluate the growth profile of L. lactis, its antibacterial activity against Escherichia coli, and to identify functional groups of active compounds using Fourier Transform Infrared (FTIR) spectroscopy. Antibacterial activity was assessed using the disk diffusion method on MHA plates inoculated with standardized E. coli suspensions. The growth curve analysis showed that L. lactis entered the lag phase between 0–9 hours, the logarithmic phase from 10–17 hours, and the stationary phase from 18–40 hours. The highest antibacterial activity was observed at 23 hours of incubation, with an inhibition zone diameter of 20 mm against E. coli. FTIR analysis of the cell-free supernatant revealed the presence of O–H, C–H, amide I, COO⁻, and C–O functional groups, suggesting the presence of bioactive compounds such as bacteriocins (e.g., nisin), lactic acid, and carbohydrate residues from the fermentation medium. These findings highlight the potential of L. lactis as a natural antimicrobial agent and support its further application in functional food development and biopharmaceutical formulations.
Studi Pengaruh Volume Tungsten terhadap Optimalisasi Biaya Proses Carbon Analyzer di PT.XYZ Setiawan, Dibyo; Handy, Rayhan Aliva Vitra; Wibowo, Cahyo; Wiyono, Apri; Putra, Fisika Prasetyo; Rinaldi, Kardian
JURNAL CRANKSHAFT Vol 6, No 3 (2023): Jurnal Crankshaft Vol.6 No.3 (2023)
Publisher : Universitas Muria Kudus

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24176/crankshaft.v6i3.11398

Abstract

PT XYZ merupakan suatu perusahaan joint venture, terdapat 2 (dua) macam proses produksi, yaitu Proses Casting dan Proses Machining, dalam proses produksi Casting, tersedia produk FC (Ferro Casting), FCD (Ferro Casting Ductile) dan dapat juga melaksanakan proses Machining, Assembling serta Metal Forming. Penghargaan yang telah diperoleh Cost Reduction Program, Good Delivery Perfomance, Good Cost Perfomance, dan Kategori Proses. Salah satu fokus terkait Cost Reduction Program bertujuan optimalisasi konsumsi dan biaya penggunaan tungsten, serta biaya tenaga kerja. Kegiatan merupakan kontribusi akademisi memberikan rekomendasi bagi PT.XYZ dalam rangka menyelesaikan masalah di Industri. Metode yang digunakan dalam pelaksanaan kegiatan optimalisasi proses carbon analyzer di PT.XYZ menggunakan pendekatan eksperimental dengan pemberlakuan volume tungsten pada setiap tahap eksperimen. Tahapan meliputi penyiapan alat, peralatan dan sampel, proses penimbangan dan pencampuran, proses treatment carbon analyzer, analisis hasil eksperimen dan kalkulasi reduksi biaya dan menyusun kesimpulan. Eksperimen yang diterapkan diantaranya volume 0, 0.75, 1.5 dan 1.8 Gram pada Treatmen Carbon Analyzer.  Hasil kegiatan diperoleh bahwa semakin banyak penggunaan volume tungsten pada proses carbon analyzer, dapat mengurangi waktu pengerjaan yang dibutuhkan oleh mesin carbon analyzer sebanyak 3 (tiga) detik. Penggunaan tungsten 0,75gram (½ dari standard) lebih efektif mampu menghemat biaya penggunaan tungsten (Cost Reduction). Selanjutnya terkait dengan penghematan, dengan menggunakan penerapan tungsten 0,75gram dapat menghemat biaya sebesar Rp 14.149.779/bulan dan penghematan biaya tenaga kerja sebesar 250.000/bulan.
The Role of Glu/Gly/Zif-8 Edible Film in Improving the Superior Rheological and Water Vapor Barrier Properties and Antibacterial Activity for Active Food Packaging Djenar, Nancy Siti; Rinaldi, Kardian; Rahma, Dina Nur; Kustiana, Dina
Chimica et Natura Acta Vol 14, No 1 (2026)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cna.v14.n1.66715

Abstract

The low barrier to water vapor and gas is a critical limitation of glycerol-plasticized gluten-based edible films (GBEF) for food packaging applications. In this study, gluten (Glu) and glycerol (Gly) were cross-linked with 0.25–1.00% (w/v) zeolitic imidazolate framework-8 (ZIF-8) to improve interfacial compatibility and enhance the structural integrity of the film matrix. The formation of hydrogen bonds and electrostatic interactions between ZIF-8 and the Glu/Gly matrix strengthened the chemical network, resulting in increased tensile strength (TS). The water vapor transmission rate (WVTR) of Glu/Gly/ZIF-8 edible films (Glu/Gly/ZIF-8 EF) ranged from 1.085 to 1.453 g/m²/24 h, indicating significantly improved barrier properties. Mechanical testing showed TS and elongation at break (EAB) values of 32.18–60.58 MPa and 211.86–382.83%, respectively, meeting Japanese Industrial Standards for edible films (TS > 0.3 MPa, EAB > 70%, WVTR < 10 g/m²/24 h, thickness < 0.25 mm). Glu/Gly/ZIF-8 EF also demonstrated strong antibacterial activity, with inhibition zones of 25–26 mm for Gram-negative Escherichia coli and 25–34 mm for Gram-positive Staphylococcus aureus. These findings demonstrate that ZIF-8 effectively enhances both the mechanical and functional performance of gluten/glycerol films, providing a promising strategy to expand their application as bioactive packaging materials in the food industry.
Co-Authors Apri Wiyono Cahyo Wibowo Handy, Rayhan Aliva Vitra Krista, Gustin Mustika Kustiana, Dina Maswanti, Azzahra Junisa Nancy Siti Djenar Putra, Fisika Prasetyo Rahma, Andien Tiara Rahma, Dina Nur setiawan, Dibyo