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Munif, Tri Haidar
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Characterization of Cellobiose Dehydrogenase (CDH) Enzyme in Trametes versicolor strain TRAMS Munif, Tri Haidar; Rahmawan, Marjori Bethania; Abdulgani, Nurlita; Prasetyo, Endry Nugroho
Jurnal Biologi Tropis Vol. 25 No. 3 (2025): Juli-September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i3.9474

Abstract

Cellobiose dehydrogenase (CDH, EC 1.1.99.18) is an enzyme classified under the oxidoreductase group. CDH has been widely utilized in various fields, including healthcare such as in wound dressing and in the energy sector for bioenergy production. To support these industrial applications, it is necessary to investigate the characteristics of CDH to enhance process efficiency. Therefore, this study aims to characterize CDH produced by the fungus Trametes versicolor strain TRAMS. The research involved constructing a CDH production curve followed by enzyme activity assays, protein content determination, isoelectric point (pI) analysis, and kinetic studies using Michaelis-Menten and Lineweaver-Burk plots. The results showed that the enzyme exhibited an activity of 3.441 U/mL on the 8th day of production. The enzyme's specific activity reached 61.57 U/mg at the 80% ammonium sulfate fraction, with an isoelectric point at pH 4. Enzyme kinetics based on the Michaelis-Menten plot revealed a Vmax of 6.7 µM/min and a Km of 3.35 µM, while the Lineweaver-Burk analysis indicated a Vmax of 9.625 µM/min and a Km of 0.23 µM.
Co-Authors Nurlita Abdulgani Rahmawan, Marjori Bethania Setyo Nugroho