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Analysis of Antioxidant Activity of Curcumin Extract from White Turmeric (Curcuma zedoria) and Yellow Turmeric (Curcuma longa) Using Soxhletation Method Vivi Shofia; Anggraeni, Adek Bela; Nurlaila, Hamida
Indonesian Journal of Science and Pharmacy Vol. 1 No. 3 (2024): Indonesian Journal of Science and Pharmacy
Publisher : Pustaka Media Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63763/ijsp.v1i3.49

Abstract

Turmeric has been a staple in traditional medicine and cuisine in various countries. Tumeric contains the main bioactive compound, namely curcumin. Curcumin is recognized as antioxidants that can which donate one or more electrons to free radicals, effectively neutralizing them. The commonly used turmeric varieties are white turmeric (Curcuma zedoaria) and yellow turmeric (Curcuma longa). White turmeric is characterized by its white rhizome, while yellow turmeric has a yellow rhizome. Both varieties contain curcumin, but their antioxidant activities must be fully elucidated. The Soxhlet method is a common technique employed for curcumin extraction. This study investigated the antioxidant activity of white turmeric and yellow turmeric compared to vitamin C. The DPPH method was used to assess antioxidant activity. The findings revealed that white turmeric (Curcuma zedoaria) and yellow turmeric (Curcuma longa) extracts exhibited antioxidant activity with IC50 values of 12.39 µg/mL and 44.065 µg/mL, respectively. Conversely, vitamin C showed an IC50 value of 19.95 µg/mL. These results indicate that yellow turmeric curcumin extract possesses superior antioxidant activity compared to white turmeric and vitamin C, falling within the category of solid antioxidants with IC50 values below 50 µg/mL.
Perbandingan Aktivitas Antioksidan Umbi dan Kulit Bawang Merah Probolinggo Menggunakan Metode 2,2-Diphenyl-1-Picrylhydrazyl Anggraeni, Adek Bela; Nurlaila, Hamida; Vivi Shofia; Azis, Fahmi Dimas Abdul
JI-KES (Jurnal Ilmu Kesehatan) Vol. 8 No. 2 (2025): JI-KES (Jurnal Ilmu Kesehatan)
Publisher : LPPM Universitas Hafshawaty Zainul Hasan Probolinggo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33006/jikes.v8i2.800

Abstract

Abstrak Bawang merah (Allium cepa L.) asal Probolinggo dikenal kaya akan senyawa bioaktif seperti flavonoid dan sulfur yang berfungsi sebagai antioksidan dan meningkatkan kesehatan. Penelitian ini bertujuan untuk membandingkan aktivitas antioksidan kulit dan umbi bawang merah menggunakan metode DPPH (2,2-diphenyl-1-picrylhydrazyl). Kulit dan umbi bawang merah diekstraksi menggunakan pelarut etanol, kemudian dilakukan pengukuran aktivitas antioksidan dengan metode DPPH untuk menentukan nilai IC50, yaitu konsentrasi yang dibutuhkan untuk mengurangi 50% radikal bebas. Kulit bawang merah menunjukkan aktivitas antioksidan lebih tinggi dibandingkan dengan umbi, dengan nilai IC50 masing-masing 6,41 µg/mL dan 16,50 µg/mL. Nilai IC50 yang lebih rendah pada kulit bawang merah menunjukkan aktivitas antioksidan yang lebih kuat dibandingkan umbi, menunjukkan perbedaan potensi antioksidan antara kedua bagian tanaman. Penelitian ini mengungkap bahwa kulit bawang merah memiliki potensi sebagai sumber antioksidan alami yang lebih tinggi dibandingkan umbi, dengan kedua bagian tanaman dikategorikan sebagai antioksidan kuat (IC50 < 50 µg/mL). Kulit bawang merah dapat dijadikan bahan alami yang lebih efektif dalam mencegah kerusakan akibat radikal bebas. Kata kunci: bawang merah, antioksidan, DPPH, kulit bawang merah, umbi bawang merah.   Abstract Probolinggo's shallot (Allium cepa L.) are well-known for being abundant in bioactive substances including sulfur and flavonoids, which have anti-oxidant and health-promoting properties. This study aims to compare the antioxidant activity of shallot skin and bulbs using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method. After ethanol solvent was used to extract the shallot skins and bulbs, the DPPH method was used to evaluate antioxidant activity and calculate the IC50 value—the concentration required to eliminate 50% of free radicals. The antioxidant activity of shallot skin was higher than that of bulbs, with IC50 values of 6.41 µg/mL and 16.50 µg/mL, respectively. Shallot skin has a lower IC50 value than bulbs, indicating higher antioxidant activity. This suggests that the two plant components have different antioxidant capacity. With both plant parts classified as powerful antioxidants (IC50 < 50 µg/mL), this study showed that shallot skin has the potential to be a higher source of natural antioxidants than bulbs. Shallot skin can be used as a natural ingredient that is more effective in preventing free radical damage. Keywords: shallot, antioxidant, DPPH, shallot skin, shallot bulb.