This research aims to analyze the level of learning difficulty of class X MAN 1 Mamuju students in odd semester biology material. The type of research is quantitative survey method research with 167 samples determined using census techniques. The research instruments are biology learning results tests, questionnaires and observation sheets. The research data was analyzed by calculating the average and percentage and then categorizing it. Based on the research results, the majority of students experienced learning difficulties out of the 167 students who were the research samples. Biology test material to determine learning difficulties consists of Biology and its Role in Life with an average score of 84, Viruses and their Role in Life with an average score of 73, Prokaryotic Organisms and their Role in Life with an average score of 63, Protista and their Role in Life with an average score of 43, Kingdom Fungi and their Role in Life with an average score of 57. The factors that cause students' learning difficulties consist of internal and external factors. The internal factor that causes learning difficulties with the highest negative answer is the fatigue factor at 76.05% and the external factor that causes learning difficulties with the highest negative answer is the community factor at 70.51%. The highest negative answer was for the sub-indicator of internal factors, namely spiritual factors, while the lowest negative answer was physical disability at 17.37%, then the sub-indicator with the highest negative answer for external factors was the form of life in society at 78.44%, while the lowest average negative answer was school time at 45.51%.