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All Journal Spizaetus: Jurnal Biologi dan Pendidikan Biologi Journal of BioSustainability
Fadhilah, Eksa Adhwa
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Other

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Pemanfaatan Limbah Sekam Padi (Oryza sativa Linn.) sebagai Media Fermentasi untuk Produksi Enzim Selulase oleh Aspergillus niger Fadhilah, Eksa Adhwa; Peristiwati; Surakusumah, Wahyu
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 2 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i2.559

Abstract

This study aims to optimize the production of cellulase enzyme by the cellulolytic fungus Aspergillus niger using rice husk powder (Oryza sativa Linn.) as substrate. Cellulase enzymes have important roles in various industrial applications, including the bioconversion of lignocellulosic waste into high-value products such as bioethanol, pulp, paper, and animal feed. The utilization of rice husk as a substrate offers economic and environmental advantages due to its abundant availability, low cost, and ability to reduce the volume of agricultural waste. The research method used a quantitative experimental approach to evaluate the effect of fermentation parameters, such as pH, temperature, and incubation time, on enzyme production. A. niger isolates were developed on carboxymethylcellulose (CMC) media to ascertain their cellulolytic capacity, with measurements of biomass, pH, temperature, and enzyme activity. The results showed that the biomass of A. niger increased from hour 0 to hour 96 at pH 4.5 to 5.5, and temperature 29.5°C to 30.5°C. The optimum condition for enzyme production was found at pH 4.5 and temperature 30.5°C, with the highest enzyme activity reaching 0.736 U/mL. These findings suggest that optimization of cellulase enzyme production from rice husk not only improves enzyme production efficiency, but also contributes to more sustainable waste management.
Bioremediation Potential of Nymphaea sp., Zantedeschia aethiopica, and Spirodela polyrhiza for Laundry Wastewater Treatment Ramadhani, Abidah Bunga; Fadhilah, Eksa Adhwa; Lestari, Emilia Mia; Azizah, Hilda Nur; Christofan, Vinno Bayu; Surakusumah, Wahyu; Dewina, Restu Utari
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Publisher : Universitas Pendidikan Indonesia and Konsorsium Biologi Indonesia

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Abstract

This research aims to assess the efficacy of three distinct types of aquatic plants, namely Nymphaea sp., Hydrocotyle sp., and Spirodela sp., in wastewater bioremediation from the laundry industry. The wastewater from the laundry industry contains a range of chemical substances that have the potential to pollute the surrounding environment. These include detergents, fragrances, and other chemicals that can cause pollution of the aquatic environment. The research method entailed the simultaneous planting of the three types of aquatic plants in controlled containers with the tested laundry wastewater. The temperature, pH, total dissolved solids (TDS), and dissolved oxygen (DO) of the laundry water effluent were measured before and after passing through the aquatic plant system. The results demonstrated that all three types of aquatic plants exhibited the capacity to reduce the concentration of TDS in laundry wastewater. The study revealed that there were notable differences in the effectiveness of various aquatic plant species in reducing TDS. Of the three plant species examined, Spirodela sp. demonstrated the greatest potential for reducing detergent concentrations, as indicated by a notable decrease in TDS.
Co-Authors Azizah, Hilda Nur Christofan, Vinno Bayu Dewina, Restu Utari Lestari, Emilia Mia Peristiwati Ramadhani, Abidah Bunga Wahyu Surakusumah