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All Journal AL KAUNIYAH Jurnal Penelitian Sains
Sari, Arina Findo
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Biochemistry, Genetics & Molecular Biology Chemistry Materials Science & Nanotechnology Mathematics Physics

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Isolasi dan Identifikasi Mikroorganisme Dari Produk Ekoenzim WOP FST 1310 Sari, Arina Findo; Eka Putri, Lily Surayya; Hariwibowo, Dinda Rama; Riliansyah, Armar; Sugoro, Irawan; Mujiyanto, Aldi; Hamada, Firdaus Ramadhan
Jurnal Penelitian Sains Vol 25, No 3 (2023)
Publisher : Faculty of Mathtmatics and Natural Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56064/jps.v25i3.876

Abstract

Produk ekoenzim Waste Organic Product (WOP) FST 1310, merupakan produk buatan tim Fakultas Sains Dan Teknologi, UIN Syarif Hidayatullah. Produk ini terbuat dari 2 jenis sampah organik yang berbeda, yaitu sisa sayur dan kulit jeruk. Penelitian mengenai konsorsium mikroba pada produk ekoenzim belum banyak dilaporkan. Pentingnya mengetahui mikroba yang hadir pada produk WOP FST 1310 merupakan langkah awal untuk menentukan pemilihan bahan substrat yang akan digunakan. Karakterisasi makroskopis diamati bentuk koloni, permukaan koloni, tepi koloni dan warna koloni. Karakter mikroskopis dilakukan dengan pengamatan bentuk sel dan pewarnaan gram. Identifikasi koloni dengan uji fisiologis biokimia yang diujikan yaitu, H2S, karbohidrat (uji katalase dan TSIA), fermentasi gula (glukosa, sukrosa, laktosa, manitol) dan MR-VP. Berdasarkan hasil diperoleh 11 isolat bakteri dan jamur dari kedua bahan dengan lima isolat dari kulit jeruk dan enam isolat dari sayuran. Berdasarkan uji makroskopis, mikroskopis, pewarnaan gram, aktivitas fisiologis dan biokimia merupakan isolat KJ NA 1, KJ NA 2, SY NA 3, SY NA 4, SY NA 5, dan SY NA 6 merupakan bakteri asam laktat (BAL).Kata kunci: bakteri, eko-enzim, jamur, WOP FST 1310
Methane Gas Production In Buffalo Rumen Fluid Containing Citronella Residue (Cymbopogon nardus L.) by In Vitro Method Sari, Arina Findo; Mangunwardoyo, Wibowo; Setiawan, Fery Hadi; Sugoro, Irawan
Al-Kauniyah: Jurnal Biologi Vol. 18 No. 2 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v18i2.40935

Abstract

Methane is one of the greenhouse gases produced by ruminants. One way to reduce methane is by feeding development strategies. Concentrates or forage are two possible forms of animal feed. Forage can be replaced with citronella (Cymbopogon nardus L.) residue, which has not been fully consumed as feed. The purpose of the study was to decide the production of methane gas that has residual citronella to concentrate in buffalo rumen fluid in vitro, for 48 hours. The treatments in this research were 0.4 g citronella residue (A); 0.4 g citronella residue + 0.01 g concentrate (B); 0.4 g citronella residue + 0.02 g concentrate (C); 0.01 g concentrate (D); and 0.02 g concentrate (E). For the 48-hour measurements, the highest methane gas production was treatment 98.2% (D); followed by 92.06% (E); 17.71% (C); 15.33% (A); and 13.54% (B). It can be concluded that methane gas can be reduced by residue citronella. This shows that citronella residue can lower methane gas. The study's findings are anticipated to be among the references for using agricultural product residues, particularly citronella as animal feed to reduce the methane produced by the livestock industry
Co-Authors Eka Putri, Lily Surayya Firdaus Ramadhan Hariwibowo, Dinda Rama Irawan Sugoro Mujiyanto, Aldi Riliansyah, Armar Setiawan, Fery Hadi WIBOWO MANGUNWARDOYO