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All Journal AL KAUNIYAH Jurnal Penelitian Sains Journal of Academic Biology and Biology Education
Sari, Arina Findo
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Biochemistry, Genetics & Molecular Biology Chemistry Education Materials Science & Nanotechnology Mathematics Physics Veterinary Other

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Isolasi dan Identifikasi Mikroorganisme Dari Produk Ekoenzim WOP FST 1310 Sari, Arina Findo; Eka Putri, Lily Surayya; Hariwibowo, Dinda Rama; Riliansyah, Armar; Sugoro, Irawan; Mujiyanto, Aldi; Hamada, Firdaus Ramadhan
Jurnal Penelitian Sains Vol 25, No 3 (2023)
Publisher : Faculty of Mathtmatics and Natural Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56064/jps.v25i3.876

Abstract

Produk ekoenzim Waste Organic Product (WOP) FST 1310, merupakan produk buatan tim Fakultas Sains Dan Teknologi, UIN Syarif Hidayatullah. Produk ini terbuat dari 2 jenis sampah organik yang berbeda, yaitu sisa sayur dan kulit jeruk. Penelitian mengenai konsorsium mikroba pada produk ekoenzim belum banyak dilaporkan. Pentingnya mengetahui mikroba yang hadir pada produk WOP FST 1310 merupakan langkah awal untuk menentukan pemilihan bahan substrat yang akan digunakan. Karakterisasi makroskopis diamati bentuk koloni, permukaan koloni, tepi koloni dan warna koloni. Karakter mikroskopis dilakukan dengan pengamatan bentuk sel dan pewarnaan gram. Identifikasi koloni dengan uji fisiologis biokimia yang diujikan yaitu, H2S, karbohidrat (uji katalase dan TSIA), fermentasi gula (glukosa, sukrosa, laktosa, manitol) dan MR-VP. Berdasarkan hasil diperoleh 11 isolat bakteri dan jamur dari kedua bahan dengan lima isolat dari kulit jeruk dan enam isolat dari sayuran. Berdasarkan uji makroskopis, mikroskopis, pewarnaan gram, aktivitas fisiologis dan biokimia merupakan isolat KJ NA 1, KJ NA 2, SY NA 3, SY NA 4, SY NA 5, dan SY NA 6 merupakan bakteri asam laktat (BAL).Kata kunci: bakteri, eko-enzim, jamur, WOP FST 1310
Unexpected Insights: Raw Sub-Bituminous Coal Outperforms Gamma-Irradiated Coal in Bio-solubilization with Phanerochaete chrysosporium Sari, Arina Findo; Rasul, Zharifa G.; Sampulna, Jamela B.
Journal of Academic Biology and Biology Education Vol. 2 No. 1 (2025): June
Publisher : Cahaya Ilmu Cendekia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37251/jouabe.v2i1.2152

Abstract

Purpose of the study: The purpose of this study is to investigate the effect of gamma irradiation treatment on sub-bituminous coal in the bio-solubilization process using Phanerochaete chrysosporium and to characterize the bio-solubilization products of raw and irradiated coal under laboratory conditions. Methodology: This study used sub-bituminous coal from South Sumatra, gamma irradiated with an Irpasena irradiator, and biosolubilized with Phanerochaete chrysosporium. Instruments included a Precision® shaker incubator, Novel® microscope, Hanna® pH meter, Shimadzu® GC-MS, and Shimadzu® UV-Vis spectrophotometer. Data were analyzed statistically using T-test with SPSS version 20 software. Main Findings: The results revealed that raw coal exhibited higher solubilization efficiency compared to irradiated coal, indicated by a greater increase in soluble phenolic (0.95 mg/mL) and aromatic compounds (1.42 mg/mL), as well as higher protein production. GC-MS analysis confirmed that raw coal generated more diverse and abundant metabolites, including phenolic and aromatic derivatives, while irradiated coal produced fewer compounds. Statistical analysis (T-test, p < 0.05) supported the significant differences between treatments. These findings suggest that gamma irradiation does not enhance, and may even inhibit, coal bio-solubilization .. Novelty/Originality of this study: This study provides new evidence that gamma irradiation does not enhance, but rather reduces, the bio-solubilization efficiency of sub-bituminous coal by Phanerochaete chrysosporium. The findings challenge the common assumption about irradiation benefits and contribute to advancing knowledge by highlighting the superior performance of raw coal in producing energy-equivalent products.
Methane Gas Production In Buffalo Rumen Fluid Containing Citronella Residue (Cymbopogon nardus L.) by In Vitro Method Sari, Arina Findo; Mangunwardoyo, Wibowo; Setiawan, Fery Hadi; Sugoro, Irawan
Al-Kauniyah: Jurnal Biologi Vol. 18 No. 2 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v18i2.40935

Abstract

Methane is one of the greenhouse gases produced by ruminants. One way to reduce methane is by feeding development strategies. Concentrates or forage are two possible forms of animal feed. Forage can be replaced with citronella (Cymbopogon nardus L.) residue, which has not been fully consumed as feed. The purpose of the study was to decide the production of methane gas that has residual citronella to concentrate in buffalo rumen fluid in vitro, for 48 hours. The treatments in this research were 0.4 g citronella residue (A); 0.4 g citronella residue + 0.01 g concentrate (B); 0.4 g citronella residue + 0.02 g concentrate (C); 0.01 g concentrate (D); and 0.02 g concentrate (E). For the 48-hour measurements, the highest methane gas production was treatment 98.2% (D); followed by 92.06% (E); 17.71% (C); 15.33% (A); and 13.54% (B). It can be concluded that methane gas can be reduced by residue citronella. This shows that citronella residue can lower methane gas. The study's findings are anticipated to be among the references for using agricultural product residues, particularly citronella as animal feed to reduce the methane produced by the livestock industry
Co-Authors Eka Putri, Lily Surayya Firdaus Ramadhan Hariwibowo, Dinda Rama Irawan Sugoro Mujiyanto, Aldi Rasul, Zharifa G. Riliansyah, Armar Sampulna, Jamela B. Setiawan, Fery Hadi WIBOWO MANGUNWARDOYO