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All Journal Journal of Indonesian Medical Laboratory and Science (JoIMedLabS)
Khusna , Umi Nihayatul
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Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology

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Identification of Salmonella Sp. in Duck Eggs Sold in Larangan Village Putri, Nadia Auditia; Khusna , Umi Nihayatul; Jaman , Anwar
Journal of Indonesian Medical Laboratory and Science Vol 6 No 1: April 2025
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v6i1.245

Abstract

Background: Duck eggs are one of the foodstuffs that are very susceptible to damage caused by microbes. The types of microbes that contaminate eggs are mainly from the Gram-negative bacteria type, including Salmonella. Objectives: The purpose of this study was to determine the salmonella sp bacteria in duck eggs sold in Larangan village Materials and Methods: The examination method used was observation of laboratory tests, isolation methods, and identification of bacteria using biochemical tests. The samples used were duck egg yolks, using Brain Heart Infusion Broth (BHIB) media, Salmonella Shigella Agar (SSA), gram staining, Triple Sugar Iron Agar (TSIA), Simon citrate, and Sulfide Indol Motility (SIM). Results: The results of the research that has been done on BHIB media obtained 6 samples showed bacterial growth, on SSA media 6 duck egg samples showed bacterial colony growth, on gram staining obtained gram-negative and rod-shaped bacteria in 6 samples, on TSIA media obtained positive results on 6 media, simon citrate test showed positive results on 6 media, SIM test on motile test 6 media obtained positive results, on sulfide test 5 media obtained positive results, while indole test 6 media obtained negative results. Conclusions: Based on the results of research on the identification of salmonella sp bacteria in duck eggs sold in Larangan village, Larangan sub-district, Brebes regency, Central Java, it was found that 6 duck egg samples contained salmonella sp bacteria.
Hubungan Personal Hygiene Dengan Kejadian Kecacingan Pada Anak Usia 6-9 Tahun Di Desa Pesarean Pagerbarang Tegal Jawa Tengah Khasanah, Isma Laelatul; Khusna , Umi Nihayatul; Jaman , Anwar
Journal of Indonesian Medical Laboratory and Science Vol 6 No 1: April 2025
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v6i1.248

Abstract

Background: Ringworm disease is one of the diseases caused by infections with Soil Transmitted Helminths (STH) which usually attacks children because they are less able to maintain Personal Hygiene such as still liking to play in the soil,and not washing hands after playing.Objectives: : The aim of this research is to determine the relationship between personal hygiene and the incidence of worms in children aged 6-9 years in Pesarean Pagerbarang Village, Tegal, Central Java.Materials and Methods: This study uses an Observational Analytical research type using a cross-sectional approach , with a Incidental Sampling technique.Results: Based on the results of the questionnaire interview, it was found that 17 children (48.57%) had good personal hygiene, and 18 children (51.43%) had poor personal hygiene. Laboratory examination showed that 21 children (60.00%) were infected with STH, including Ascaris Lumbricoides, 18 children (85.71%). Tricuris Trichiura 1 child (4.76%), Necator Americaanus 2 children (9.52%). Conclusions: The results of analysis using chi-square obtained p-value = 0.000 for the relationship between personal hygiene and the incidence of worms and p-value = 0.013 for the relationship between nail cleanliness and the incidence of worms. This shows that there is a significant relationship between the two because ?<0.05. Keywords Incidence of worms; Personal hygiene..
Co-Authors Jaman , Anwar Khasanah, Isma Laelatul Putri, Nadia Auditia