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All Journal Jurnal Teras Kesehatan Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) Health Research Journal of Indonesia
Khusna, Umi Nihayatul
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Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Physics Public Health

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Identifikasi bakteri Escherichia coli dan Salmonella sp. pada Spons Pencuci Piring Berdasarkan Lama Penggunaan Turrohmah, Alhidayah; Khusna, Umi Nihayatul; Jaman , Anwar
Journal of Indonesian Medical Laboratory and Science Vol 6 No 1: April 2025
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v6i1.246

Abstract

Background: Sponges are tools used to clean cutlery. Dishwashing sponges are the main source of bacterial cross-contamination because they can transmit foodborne pathogens. Sponges that are used for a long time will have more potential to grow various microorganisms, such as bacteria from the Enterobacteriaceae family, including Escherichia coli and Salmonella sp. Objectives: The purpose of this study was to identify Escherichia coli, Salmonella sp..and the proper duration of use on sponges to avoid bacterial contamination. Materials and Methods: The type of research is descriptive, with a Quota sampling technique with a sample size of 6 dishwashing sponges at 6 points in the house (2 samples at one time), which have been determined on three indicators, namely 3,5,7 weeks of use. Observation and testing data collection methods. Results: The results showed negative Esherichia coli bacteria in all sponge samples with a duration of use of 3, 5, and 7 weeks, while positive Salmonella sp. bacteria in 1 sponge sample with a duration of use of 7 weeks. Conclusions: There were Salmonella sp. bacteria in 1 sample, namely a sponge used for 7 weeks and no Escherichia coli bacteria were found in all samples, all samples of dishwashing sponges with a usage period of 3, 5, and 7 weeks tested positive for bacterial contamination, so the minimum usage period for the sponge is 2 weeks, to avoid bacterial contamination from the dishwashing sponge.
Hubungan Kejadian Hiperglikemia dengan Hipertensi pada Peserta Posyandu Lansia di Desa Kubangsari Brebes Casyanti, Casyanti; Khusna, Umi Nihayatul
JURNAL TERAS KESEHATAN Vol 9 No 1 (2026): Jurnal Teras Kesehatan
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Politeknik Al Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.38215/6ekzwd46

Abstract

Hyperglycemia and hypertension are two common health disorders among the elderly. Persistently elevated blood glucose levels in individuals with diabetes mellitus can lead to various health complications, one of which is hypertension. Therefore, this study aimed to determine whether there is an association between hyperglycemia and hypertension among elderly individuals attending the integrated healthcare post (Posyandu) in Kubangsari Village, Ketanggungan District, Brebes Regency, in 2025. This study employed an analytic observational design with a cross-sectional approach. A total of 80 respondents were included as samples. Random blood glucose levels were measured using a point-of-care testing (POCT) device, while blood pressure was measured using a sphygmomanometer. Quantitative data analysis was performed using the Chi-square test. The results showed that 61 respondents (76.3%) had normal blood glucose levels, and 34 respondents (42.5%) had normal blood pressure. The Chi-square analysis indicated a significance value of p < 0.001, suggesting a statistically significant association between hyperglycemia and hypertension. Based on these findings, it can be concluded that hyperglycemia contributes to the occurrence of hypertension among the elderly. Therefore, routine health examinations are necessary to prevent further complications.
Analisis Pertumbuhan Dan Pola Hemolisis Bakteri Staphylococcus aureus Pada Media Darah Kambing Jawa Dan Agar Darah Domba Janah, Nur Intan Raodlotul; Khusna, Umi Nihayatul
Health Research Journal of Indonesia Vol 4 No 4 (2026): Health Research Journal of Indonesia
Publisher : CV. Wadah Publikasi Cendekia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63004/hrji.v4i4.1048

Abstract

Blood agar merupakan media kultur mikrobiologi yang digunakan untuk mengamati pertumbuhan serta pola hemolisis bakteri, khususnya Staphylococcus aureus. Media ini umumnya menggunakan darah domba karena mampu menunjukkan pola hemolisis dengan jelas. Namun, keterbatasan ketersediaan dan tingginya biaya di Indonesia menjadi kendala dalam penggunaannya. Oleh karena itu, darah kambing Jawa berpotensi digunakan sebagai alternatif karena ketersediaannya yang melimpah di Indonesia, mudah diperoleh, serta lebih ekonomis. Penelitian ini menggunakan desain eksperimental dengan dua perlakuan dan tiga kali pengulangan. Tahapan meliputi defibrinasi darah, pembuatan media, inokulasi Staphylococcus aureus (ATCC 25923), serta pengamatan morfologi koloni, jumlah koloni, pola hemolisis, dan diameter zona hemolisis. Hasil menunjukkan morfologi koloni seragam (circular, tepi entire, elevasi convex) dan 100% isolat menghasilkan hemolisis β pada kedua media. Analisis statistik menggunakan uji Mann-Whitney menunjukkan nilai signifikansi sebesar 0,487 pada jumlah koloni dan 0,637 pada diameter zona hemolisis (p > 0,05), yang menunjukkan tidak terdapat perbedaan signifikan antara kedua media. Dengan demikian, darah kambing Jawa memiliki kemampuan sebanding dengan darah domba dalam mendukung pertumbuhan dan aktivitas hemolisis S. aureus, sehingga berpotensi sebagai alternatif media blood agar yang lebih ekonomis.
Co-Authors Casyanti, Casyanti Jaman , Anwar Janah, Nur Intan Raodlotul Turrohmah, Alhidayah