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Iwataki, Mitsunori
Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)
Earth & Planetary Sciences

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THE PHYLOGENETIC TREE OF Alexandrium Prorocentrum AND PSEUDO-NITZSCHIA OF HARMFUL AND TOXIC ALGAE IN VIETNAM COASTAL WATERS BASED ON SEQUENCES OF 18sRdna, Its1-5.8s-Its2 GENE FRAGMENTS AND SINGLE CELL -PER METHOD Hong, Dang Diem; Thu, Ngo Thi Hoai; Nam, Hoang Sy; Hien, Hoang Minh; Hai, Luyen Quoc; Ha, Dao Viet; Fukuyo, Yasuo; Iwataki, Mitsunori
Marine Research in Indonesia Vol 32, No 2 (2007)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/mri.v32i2.456

Abstract

Molecular biological techniques support the identification of microalgae of Vietnam. Prorocentrum, Alexandrium and Pseudo-nitzschia are main harmful and toxic microalgal genera found in Vietnam coastal waters. The results of morphology and nucleotide sequence analysis of 18S rDNA and ITS 1-5.8S-ITS2 gene obtained from genomic DNA have shown that the Prorocentrum sp. 3 (isolated from Cat Ba, Hai Phong on October, 2004), Alexandrium sp. 5 (collected on October, 2004) and Pseudo-nitzschia sp. G3 (colleted in Do Son, Hai Phong on December, 2005) belonging to Prorocentrum mexicanum (the homological percent of 99.9% with sequencing of P mexicanum in Genbank ofY16232, AY886763), A. minutum (99.8% - AJ535388, DQ168664) and Pseudo-nitzschia pungens (98.8% - AY544769, DQ166533), respectively. The obtained results indicated that the exceptional fresh samples, for Prorocentrum genus which may be preserved at 25% ethanol, 4% formaldehyde, 1% glutaraldehyde, while Alexandrium genus ó at 25% ethanol, and Pseudo-nitzschia genus - 4% formaldehyde and 1% glutaraldehyde for two weeks have not effected on their analysis of sequences generated by Single Cell PCR method. In these studied samples, the nucleotide sequences obtained from genomic DNA and Single-cell PCR methods were the same with the homological percent more than 99%. Application of this method to samples collected from Phu Quoc Island, southern part of Vietnam, in 27-29, June, 2006 showed that Prorocentrum mican and P sigmoisdes were found.
MORPHOLOGY OF A NEW HETEROCAPSA SPECIES (PERIDINIALES, DINOPHYCEAE) OCCURED IN HUE, VIETNAM Iwataki, Mitsunori; Kawami, Hisae; Nguyen, Nguyen van; Doc, Luong Quang; Phap, Ton That; Fukuyo, Yasuwo; Matsuoka, Kazumi
Marine Research in Indonesia Vol 32, No 2 (2007)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8529.232 KB) | DOI: 10.14203/mri.v32i2.448

Abstract

To investigate the distribution of Heterocapsa including a harmful species H. circularisquama, cells were surveyed in three Vietnamese coasts, Ha Long Bay, Hue and Phu Quoc Island in 2006. Cells of Heterocapsa were detected from Lang Co Lagoon and adjacent coast in Hue. Cell of the species possesses thecal plates corresponding to the typical of Heterocapsa. Under TEM, body scales approximately 500 nm in diameter are observed. The scale structure is similar to that of H. illdefina, however, number of the marginal spine is different. We concluded it is an undescribed Heterocapsa species possessing a new body scale ultrastructure.
Co-Authors Doc, Luong Quang Fukuyo, Yasuo Fukuyo, Yasuwo Ha, Dao Viet Hai, Luyen Quoc Hien, Hoang Minh Hong, Dang Diem Kawami, Hisae Matsuoka, Kazumi Nam, Hoang Sy Nguyen, Nguyen van Phap, Ton That Thu, Ngo Thi Hoai