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All Journal Biospecies Jurnal SOLMA
Bonnu, Christin H.
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Other

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In Silico Identification of Putative miRNAs Targeting GME to Increase Ascorbic Acid in Tomato Bonnu, Christin H.; Tiara Ayu Nugraha Putri; Blegur, Ernes Josias
Biospecies Vol. 18 No. 2 (2025): Juli 2025
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v18i2.44411

Abstract

The primary natural sources of ascorbic acid or vitamin C are fruits and vegetables, withtomatoes being one of the most readily available and accessible examples. The biosynthesisof ascorbic acid through the L-galactose pathway plays a key role in regulating vitamin C production in tomatoes. microRNAs (miRNAs) are among the various regulators that interact with mRNA involved in this process. Investigating how miRNA regulates mRNA could provide crucial information for developing genetic engineering techniques to boost vitamin C production. The main purpose of this research is to identify potential miRNA regulators ofGME using in silico approach. The research findings demonstrated that ascorbic acid biosynthesis in tomato involves 19 gene IDs. It also discovered that GME interacts with multiple miRNAs in tomatoes, specifically sly-miR-159b, sly-miR-159a, sly-miR-167, sly-miR-319c, sly-miR-319b and sly-miR-319a. Among these miRNAs, sly-miR-159a displays higher expression, which allows it to exert a suppressive effect on GME activity (negative regulator), leading to a decrease in vitamin C production. Decreasing the expression of this miRNA provides a potential molecular approach for enhancing the nutritional quality of tomato cultivars in response to the rising demand for functional foods.
Diversifikasi Produk Pertanian Melalui Pelatihan Pembuatan Sabun Batang Organik Bonnu, Christin H.; Pandu, Marvin Jecson; Karo-Karo, Esra Frandika
Jurnal SOLMA Vol. 14 No. 3 (2025)
Publisher : Universitas Muhammadiyah Prof. DR. Hamka (UHAMKA Press)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/solma.v14i3.20978

Abstract

Pendahuluan: Budidaya pertanian merupakan aktivitas ekonomi utama anggota kelompok tani, namun hasil panen umumnya dimanfaatkan dalam bentuk mentah sehingga memiliki nilai ekonomi terbatas. Kegiatan pengabdian masyarakat ini bertujuan memperkenalkan diversifikasi usaha melalui produksi sabun batang organik skala kecil untuk meningkatkan nilai produk dan mengembangkan keterampilan kewirausahaan bagi anggota kelompok tani. Selain itu, kegiatan ini juga bertujuan menghasilkan sabun organik serta mengevaluasi karakteristik dan tingkat penerimaan produknya. Metode: Pelaksanaan kegiatan mengkombinasikan ceramah singkat dan praktik langsung pembuatan sabun batang organik. Hasil: Produk sabun yang dihasilkan memiliki pH 7 (sesuai standar SNI) dengan warna krem. Tingkat kesukaan peserta terhadap aspek warna sebesar 3,5 dari 4, aroma 3,4, tekstur 3,3, busa 3,7, kebersihan 3,4, dan keseluruhan 3,5 (kategori sangat suka). Kesimpulan: Kegiatan ini menghasilkan keterampilan baru bagi kelompok tani serta produk ramah lingkungan dengan potensi ekonomi yang menjanjikan.
Co-Authors Blegur, Ernes Josias Karo-Karo, Esra Frandika Pandu, Marvin Jecson Putri, Tiara Ayu Nugraha