The thermal behavior of the single-axis tracked bifacial photovoltaic (PV) module is important for efficient energy extraction in large-scale power plants, especially in tropical regions under high irradiation and high ambient temperature. However, it is difficult to accurately predict their operating temperature due to the complex interaction between environmental variables and the characteristics of solar tracking. The available models, ranging from empirical correlations and computational fluid dynamics (CFD) simulations to machine learning methods, face challenges in terms of accuracy, interpretability, and computational load. This gap is addressed in this study, with the development of a modeling methodology based on symbolic regression (SR) utilizing genetic algorithms (GA) towards obtaining an explicit, interpretable Equation for the prediction of the PV module temperature in single-axis tracking systems. One year of data was collected at 5-minute intervals from a 19.9 MW PV plant located in San Marcos, Colombia, consisting of measurements for solar radiation, ambient temperature, wind speed, and module temperature. The constructed SR GA model achieved satisfactory prediction accuracy compared to classic models with the best root mean square error (RMSE = 4.14 °C) and R² (0.91) on the test data set. These results compare favorably with results from MLR (RMSE = 4.31 °C, R² = 0.90), the standard industry NOCT model (RMSE = 8.59 °C, R² = 0.60), and the empirical Skoplaki I model (RMSE = 5.92 °C, R² = 0.81). The resulting symbolic equation directly characterizes the effects of nonlinear solar radiation, ambient temperature, and wind speed, providing greater physical insight into the thermal dynamics of the system. An important finding is that the maximum temperature of the bifacial module is reached around 14:00h, probably due to the accumulation of temperature caused by solar tracking, which contrasts with what occurs in fixed-tilt monofacial technology. This study demonstrates that the symbolic regression technique with a genetic algorithm kernel can produce accurate, interpretable, and computationally economical models for advanced photovoltaic systems.