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Mastiadji, Bekti
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Overview of Immature Reticulocyte Fraction (IRF) and Red Cell Distribution Width-Coefficient Variation (RDW-CV) in Patients with Hemoglobin Levels Below 8 Nurhayati, Nurhayati; Mastiadji, Bekti
INDOGENIUS Vol 4 No 3 (2025): INDOGENIUS
Publisher : Department of Publication of Inspirasi Elburhani Foundation Desa. Pamokolan, Kecamatan Cihaurbeuti, Kabupaten Ciamis, Provinsi Jawa Barat, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56359/igj.v4i3.631

Abstract

Background & Objective: Anemia is a condition in which the number or volume of erythrocytes or red blood cells and hemoglobin (Hb) molecules in the body are reduced. This condition occurs when hemoglobin levels or hematocrit (Ht) values decrease. Anemia can occur due to several factors, such as iron deficiency, vitamin B12 deficiency, folic acid deficiency, genetic factors, chronic diseases, intestinal worms, and bleeding. This study aims to determine the profile of immature reticulocyte fraction (IRF) and red cell distribution width-coefficient variation (RDW-CV) in patients with hemoglobin levels below 8. Method: This type of research is descriptive research, using purposive sampling with 30 respondents. Result: The results of this study obtained IRF RDW values -CV of 15 with a percentage of (50%), normal IRF RDW-CV of 10 with a percentage of (33%), normal IRF RDW-CV of 2 with a percentage of (7%), high IRF RDW-CV of 2 with a percentage of (7%), and low IRF RDW-CV of 1 with a percentage of (3%). Conclusion: The results showed that high IRF and high RDW-CV 15 with a percentage of 50% indicated a more active bone marrow response in producing new red blood cells in response to anemia and the presence of cell size differences known as anisocytosis.
Overview of High Fluorescence Ratio (HFR), Middle Fluorescence Ratio (MFR), Mean Cell Volume (MCV), and Mean Cell Hemoglobin (MCH) in Patients with Hemoglobin Levels Below 8 Lestari, Nurul Wahidah; Mastiadji, Bekti
INDOGENIUS Vol 4 No 3 (2025): INDOGENIUS
Publisher : Department of Publication of Inspirasi Elburhani Foundation Desa. Pamokolan, Kecamatan Cihaurbeuti, Kabupaten Ciamis, Provinsi Jawa Barat, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56359/igj.v4i3.635

Abstract

Background & Objective: Anemia occurs when a person has a deficiency of red blood cells or hemoglobin, with the threshold for anemia defined as hemoglobin levels below 12 g/dl. This condition is commonly found in medical practice, especially anemia caused by iron deficiency, vitamin B12 deficiency, folate deficiency, chronic disease, or bleeding. This study aimed to describe the high fluorescence ratio (HFR), middle fluorescence ratio (MFR), mean cell volume (MCV), and mean cell hemoglobin (MCH) in patients with hemoglobin levels below 8. Method: This was a descriptive study using purposive sampling, with a total of 30 respondents. Result: The results showed that 9 patients (30%) had high HFR and MFR with normal MCV and MCH; 6 patients (20%) had normal HFR and MFR with normal MCV and MCH; 5 patients (17%) had high HFR and MFR with low MCV and MCH; 3 patients (10%) had high HFR, normal MFR, and low MCV and MCH; 2 patients (7%) had high HFR and MFR with normal MCV and low MCH; 2 patients (7%) had normal HFR and MFR with low MCV and normal MCH; 1 patient (3%) had normal HFR and MFR with low MCV and MCH; 1 patient (3%) had normal HFR, high MFR, with low MCV and normal MCH; and 1 patient (3%) had normal HFR, low MFR, and normal MCV and MCH. Conclusion: This study indicates that most patients with severe anemia (Hb < 8) showed a relatively good regenerative response (as indicated by high HFR and MFR), although variations in anemia types were also observed based on MCV and MCH values, reflecting possible different causes and classifications of anemia.
Profile of Reticulocyte Hemoglobin Equivalent (Ret-He) and Red Cell Distribution Width Coefficient of Variation (RDW-CV) in Patients with Hemoglobin Levels Below 8 Nafilah, Putri Anjani; Mastiadji, Bekti
INDOGENIUS Vol 4 No 3 (2025): INDOGENIUS
Publisher : Department of Publication of Inspirasi Elburhani Foundation Desa. Pamokolan, Kecamatan Cihaurbeuti, Kabupaten Ciamis, Provinsi Jawa Barat, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56359/igj.v4i3.636

Abstract

Background & Objective: Anemia is defined as a reduction in the concentration of hemoglobin within red blood cells (erythrocytes). It occurs when hemoglobin levels fall below 12 g/dL. Examination of Reticulocyte Hemoglobin Equivalent (Ret-He) and Red Cell Distribution Width Coefficient of Variation (RDW-CV) in patients with hemoglobin levels below 8 can assist in diagnosing and monitoring various medical conditions such as anemia and erythrocyte disorders. This study aimed to describe the Ret-He and RDW-CV profiles in patients with hemoglobin levels below 8 g/dL.   Method: This research employed a descriptive design with purposive sampling, involving 30 samples. Result: The study revealed that among 30 samples, 1 patient (3%) had high Ret-He and high RDW-CV, 4 patients (13%) had normal Ret-He and high RDW-CV, 3 patients (10%) had low Ret-He and normal RDW-CV, 20 patients (67%) had low Ret-He and high RDW-CV, and 2 patients (7%) had normal Ret-He and normal RDW-CV. Conclusion: The highest proportion, 20 patients (67%), showed low Ret-He and high RDW-CV. This finding indicates that the bone marrow response was less active, resulting in immature red blood cells lacking sufficient iron content, thereby limiting normal hemoglobin synthesis in reticulocytes. Furthermore, the presence of increased RDW-CV reflects anisocytosis, or variation in red blood cell size.
Co-Authors Lestari, Nurul Wahidah Nafilah, Putri Anjani Nurhayati Nurhayati