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All Journal Caraka Tani: Journal of Sustainable Agriculture Genbinesia Journal of Biology
Kurnia, Methodius Digna
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Health Professions Immunology & microbiology

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The Molecular and developmental insights into gonadogenesis and primordial germ cell migration in pacific white shrimp (Litopenaeus vannamei): A comprehensive review Hananya, Ariel; Iman, Mohamad Nurul; Rifany, Ade; Kusuma, Satria Aji; Kurnia, Methodius Digna; Widodo, Rubiyanto; Efendy, Hendi Yanto; Jacinda, Adinda Kinasih; Akiyama, Dean Mikio; Laiman, Hendri
Genbinesia Journal of Biology Vol. 3 No. 3 (2024): July 2024
Publisher : Generasi Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55655/genbinesia.v3i3.64

Abstract

The Pacific white shrimp, Litopenaeus vannamei, is a key species in global aquaculture, valued for its rapid growth and high market demand. Understanding the molecular mechanisms of gonadogenesis and primordial germ cell (PGC) migration in this species is vital for optimizing breeding programs. This review consolidates current knowledge on these processes, highlighting the role of the vasa gene as a germ cell marker. Vasa expression was tracked from fertilized eggs to juveniles aged four months, revealing the conserved DEAD-box helicase motif essential for germ cell development. Early detection of vasa messenger ribonucleic acid (mRNA) in developmental stages such as nauplius and zoea indicates its role in germ cell specification. By the post-larval stages, PGCs formed clusters associated with somatic gonadal precursor cells (SGPs), which support PGC migration and development. Quantitative polymerase chain reaction (qPCR) and reverse transcription PCR (RT-PCR) analyses demonstrated dynamic changes in vasa gene expression, suggesting critical windows for PGC proliferation and differentiation. Comparative studies with other species, such as Macrobrachium rosenbergii and Fenneropenaeus chinensis, emphasize the evolutionary conservation of germ cell development mechanisms. These insights are crucial for enhancing reproductive management and sustainability in shrimp farming, facilitating targeted interventions to improve breeding efficiency and hatchery success.
Jakaba Undercover: Taxonomic Riddle and Potency in Indonesian Agriculture Fadilah, Risya Ayudya; Kurnia, Methodius Digna; Putra, Ivan Permana
Caraka Tani: Journal of Sustainable Agriculture Vol 39, No 2 (2024): October
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v39i2.89049

Abstract

Excessive use of chemical fertilizers leads to serious environmental and health issues, while organic biofertilizers offer a sustainable solution. Jakaba or “jamur keberuntungan abadi,” a local liquid organic fertilizer derived from fungi, is increasingly used by Indonesian farmers. However, its taxonomy and potential applications require further investigation. Moreover, the effects of jakaba on Fusarium and its impact on maize growth remain unexplored. This study aims to characterize jakaba comprehensively, evaluate its anti-Fusarium properties, conduct pathogenicity tests, and assess its effects on maize growth. Morphological analysis of fresh jakaba fruiting bodies was conducted, and molecular identification was performed based on the internal transcribed spacer (ITS) 1/4 regions. The antagonistic test was done using plant pathogenic fungi (Fusarium sp.). In addition, jakaba was evaluated for its impact on the vegetative growth of maize. Observations identified jakaba as belonging to the genus Corallomycetella, characterized by coral-shaped fruiting bodies with an orange hue and a white tip. The hyphae are septate, spore hyaline, and ellipsoid. The Basic Local Alignment Search Tool (BLAST) analysis revealed that jakaba was Corallomycetella repens, with a query cover of 99% and a phylogenetic tree 96% bootstrap (BS) value. Jakaba exhibits antibiosis activity against Fusarium sp., with an inhibition rate of 5.64%. Although C. repens has been previously identified as a cause of root rot in Indonesia, the current study reveals that jakaba is not pathogenic to maize. Furthermore, the application of jakaba’s liquid organic fertilizer at a concentration of 40 ml l-1 significantly increased plant height, leaf length, leaf width, and stem diameter compared to other treatments. These findings highlight jakaba potential as a biofertilizer.
Co-Authors Akiyama, Dean Mikio Efendy, Hendi Yanto Fadilah, Risya Ayudya Hananya, Ariel Iman, Mohamad Nurul Ivan Permana Putra Jacinda, Adinda Kinasih Kusuma, Satria Aji Laiman, Hendri Rifany, Ade Widodo, Rubiyanto