Article Per Year (5 Year)
p-Index From 2021 - 2026
0.444
P-Index
This Author published in this journals
All Journal Indonesian Journal of Biotechnology Journal of Medical Genetics and Clinical Biology (JMGCB)
Mahdi, Oras Abdulsayed
Unknown Affiliation
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
DETECTION OF CRYPTOSPORIDIUM SPECIES IN CATTLE AND HANDLERS BY MOLECULAR TECHNIQUE IN WASIT PROVINCE Hasan, Murtadha Kadhim; Mohan, Zahraa Hamid; Saeed, Farah Mohammed; Mahdi, Oras Abdulsayed; Abdulhussein, Rasha Hamzah; hamzah, Sarah Hussein; Kareem, Mustafa basim
Journal of Medical Genetics and Clinical Biology Vol. 1 No. 3 (2024): Journal of Medical Genetics and Clinical Biology
Publisher : PT. Antis International Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61796/jmgcb.v1i3.377

Abstract

I collected 300 cow feces samples (130 males - 170 females) and used molecular analysis to detect the parasite. The results showed significant differences (p ≤ 0.05) between the infection rate using particle technology of 38% (38/100) and 41% (41/100) in cows and breeders, respectively, while traditional microscopic methods showed 21% (21/100) and 17% (17/100) in cows and breeders, respectively. DNA sequence analyzes of 10 isolates from infected cows identified four species of the Cryptosporidium parasite genus which included: C. parvum (6/10), C. andersoni (2/10), C. bovis (1/10) and C. ryanae (1/10. The species C. andersoni, C. parvum, C. bovis and C. ryanae have been genetically confirmed through DNA sequence analysis, and it is considered the first recording of these species in local cattle in Iraq. In breeders, three types of the Cryptosporidium parasite were recorded through DNA sequence analysis in 10 isolates from infected individuals, which included: C. parvum (2/10), C. andersoni (5/10) and C. hominis (3/10). It is considered the first recording of this species among breeders in Iraq.
Metagenomic detection and biochemical characterization of a Streptomyces tyrosinase from Mesopotamian marsh soils: Implications for phenolic turnover Altaee, Nadia; Mahdi, Oras Abdulsayed; Sadeq, Farah Mohammed Saeed
Indonesian Journal of Biotechnology Vol 30, No 4 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.109403

Abstract

Wetland soils in southern Iraq store large carbon pools and contain abundant phenolics that can modulate microbial decomposition. In this study, we investigate bacterial tyrosinases (TYRs), type III copper enzymes that oxidize mono‐ and diphenols, in Mesopotamian marsh soils using a combined metagenomic and biochemical approach. Degenerate primers targeting conserved CuA/CuB motifs recovered diverse partial tyr fragments affiliated with Proteobacteria and Actinobacteria. From one sample, we amplified the full melC operon from a Streptomyces lineage; expressed the tyrosinase in E. coli; and purified the enzyme (SZTYR). SZTYR displayed an alkaline pH optimum (~9); retained activity up to ~70 °C; and preferentially oxidized diphenols (e.g., L‐DOPA, dopamine) over monophenols. The enzyme also acted on phenolics relevant to peat/wetland matrices (e.g., caffeic, protocatechuic, p‐coumaric and gallic acids). The results document TYR genetic diversity in Iraqi marsh soils and establish the biochemical profile of an alkaline‐adapted Streptomyces tyrosinase. While ecosystem‐level impacts were not measured, our findings motivate field‐scale assessments of in situ TYR activity, phenolic pools and oxygen/pH dynamics to evaluate potential consequences for phenolic turnover and carbon cycling in aridifying wetlands.
Co-Authors Abdulhussein, Rasha Hamzah Altaee, Nadia hamzah, Sarah Hussein Hasan, Murtadha Kadhim Kareem, Mustafa basim Mohan, Zahraa Hamid Sadeq, Farah Mohammed Saeed Saeed, Farah Mohammed