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All Journal Journal of Medical Genetics and Clinical Biology (JMGCB)
Jiyad, Nawras Saadoun Majbas
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Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing

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INDUCTION CALLUS OF MORINGA PLANT (MORINGA OLEIFERA) IN VITRO Abdul-Sadah, Zahraa Abbas trad; Ahmed, Rahma Abd Al gabar; Fadhel, Sarah Salam Talib; Ibrahim, Mohammed Allawi Abed; Jiyad, Nawras Saadoun Majbas
Journal of Medical Genetics and Clinical Biology Vol. 1 No. 8 (2024): Journal of Medical Genetics and Clinical Biology
Publisher : PT. Antis International Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61796/jmgcb.v1i8.835

Abstract

This study aims to find an easy and efficient way to induce callus tissue of (Moringa oleifera) in vitro. The seeds were used as explant for cultivation in vitro, these parts were sterilized with sodium hypochlorite (Naocl) at concentrations (0.0, 0.5, 1.0, 2.0%) for a period of time (5,10 ,15, 20 minutes) and mercury chloride solution 0.06,0.07,0.08 0.1 mg/L) and for a period of time (2,3,4,5 minutes). Explant were planted in the medium (Murashige and Skoog (MS) in addition to different concentrations of plant growth regulators (BA, NAA), either individually or in combination. The results showed that the use of sodium hypochlorite solution (NaocL) with a concentration of 2% and a sterilization period of 20 minutes gave the highest sterilization rate of 100% without causing damage to the plant part. Also, the seeds produced callus after 48 days of culture on Ms medium containing the best combination of plant growth regulators (BA 4.0, NAA 0.5 mg/L)
Co-Authors Abdul-Sadah, Zahraa Abbas trad Ahmed, Rahma Abd Al gabar Fadhel, Sarah Salam Talib Ibrahim, Mohammed Allawi Abed