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All Journal Makara Journal of Science Journal of Medical Genetics and Clinical Biology (JMGCB)
Abdullah, Iman Tajer
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Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing

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GENETIC VARIATION OF HOUSEKEEPING GENES IN MULTIDRUG RESISTANT PSEUDOMONAS AERUGINOSA Hassan, Baraa Adnan; Hamada, Yasir Hamad; Abdullah, Iman Tajer
Journal of Medical Genetics and Clinical Biology Vol. 1 No. 10 (2024): Journal of Medical Genetics and Clinical Biology
Publisher : PT. Antis International Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61796/jmgcb.v1i10.986

Abstract

Background: Pseudomonas aeruginosa is a prominent opportunistic pathogen responsible for nosocomial infections, particularly among immunocompromised individuals. Specific Background: Its ability to develop multiple antibiotic resistance poses a significant clinical challenge, highlighting the need for a deeper understanding of its genetic diversity and virulence factors. Knowledge Gap: While previous studies have explored antibiotic resistance mechanisms, there is limited research on the genetic diversity of Pseudomonas aeruginosa isolates in specific geographic regions, such as Kirkuk. Aims: This study aimed to investigate the genetic diversity of Pseudomonas aeruginosa isolates from clinical samples obtained from Kirkuk Civil Hospitals, utilizing Multi-Locus Sequence Typing (MLST) for genetic analysis. Results: Fourteen P. aeruginosa isolates were confirmed through biochemical tests and the VITEK-2 system, with an alarming 85.71% (12/14) exhibiting antibiotic resistance. Molecular analysis revealed the presence of several housekeeping genes, although some genes did not amplify. Notably, two new serotypes (PS3:id:9797 and PS4:id:9796) were identified and added to the MLST database, along with three new genes registered in NCBI. Phylogenetic analysis indicated a divergent cluster among three isolates. Novelty: This research contributes new insights into the genetic diversity of Pseudomonas aeruginosa, identifying novel serotypes and genes, which are critical for understanding its epidemiology and resistance mechanisms. Implications: The findings underscore the importance of ongoing surveillance of Pseudomonas aeruginosa in clinical settings to inform treatment strategies and public health policies aimed at managing antibiotic resistance and improving patient outcomes.
In Vitro and In Silico Analysis of the Antibacterial Activity of Bioactive Compounds Extracted from Pineapple Peel Sadik, Wafa Mohammad; Abdullah, Iman Tajer; Alnajar, Lana Abed
Makara Journal of Science Vol. 30, No. 1
Publisher : UI Scholars Hub

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Abstract

Antibiotic resistance is a critical global challenge that requires the discovery of alternative therapeutic agents. Plants -derived compounds, such as those found in Ananas comosus (pineapple)peel, offer promising antibacterial potential. This study aimed to evaluate the antibacterial activity of pineapple peel extract using both in vitro and in silico approaches. Ethanolic extracts of pineapple peel were fractionated using silica gel chromatography, and the resulting fractions were analyzed by GC-MS. Antibacterial activity was tested against Gram-positive and Gram-negative pathogens using the agar well diffusion method, and the inhibition zones were recorded. Molecular docking was performed to assess the interaction of identified compounds with the CoA-bound OdaA protein of Pseudomonas aeruginosa (PDB ID:7BOR). Fractions 3 and 4 exhibited the strongest antibacterial activity, producing inhibition zones up to 23 mm against P. aeruginosa at 50 mg/ml. GC-MS identified several bioactive compounds, among which 4-Methoxyphenylglyoxal hydrate showed the highest docking affinity (-5.56 Kcal/mole) and formed two hydrogen bonds with the bacterial target protein. These findings demonstrate that pineapple peel, often considered agricultural waste, is a valuable source of bioactive antibacterial agents. The strong in vitro activity combined with promising in silico binding results highlights its potential in developing novel treatments, particularly against Gram-negative pathogens
Co-Authors Alnajar, Lana Abed Hamada, Yasir Hamad Hassan, Baraa Adnan Sadik, Wafa Mohammad