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All Journal JURNAL KESEHATAN LINGKUNGAN (Journal of Environmental Health) Denta: Jurnal Kedokteran Gigi
Pratiwi Soesilawati
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Dentistry Public Health

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In Vivo Evaluation of Ecoenzyme as a Natural Bioinsecticide Against Tenebrio Molitor Larvae Pratiwi Soesilawati; Rafael Gerrard; Yoakim Leok Taboy; Agatha Sherly Ivana Dewi; Aurelia Graceilla Putri Nindyatama; Moses Amadeus Soetanto
JURNAL KESEHATAN LINGKUNGAN Vol. 17 No. 4 (2025): JURNAL KESEHATAN LINGKUNGAN
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jkl.v17i4.2025.343-350

Abstract

Introduction: Organic kitchen waste fermented into eco-enzyme contains organic acids and secondary metabolites with insecticidal potential. We hypothesized that intermediate dilutions would yield higher larval mortality than very low or undiluted solutions by maximizing uptake while limiting rapid deterrence or detoxification responses. Methods: Eco-enzyme was produced from kitchen residues and brown sugar through 90-day fermentation and confirmed to contain acetic acid, lactic acid, flavonoids, and alkaloids (pH 3.0). A randomized design tested five dilutions (0%, 25%, 50%, 75%, 100%) and a chemical insecticide control (Curacron 500 EC). Each treatment had three replicates of 10 Tenebrio molitor larvae (n=180). Solutions were sprayed twice daily for seven days, and mortality recorded before each application. Data were analyzed using Shapiro–Wilk tests and one-way ANOVA (α=0.05). Results and Discussion: Phytochemical screening verified organic acids and bioactive metabolites. By Day 7, cumulative mortality was: control 23.33%, 25% eco-enzyme 46.67%, 50% 13.33%, 75% 30.00%, 100% 33.33%, and chemical insecticide 86.67%. The 25% group showed normal distribution and significantly higher mortality than several other eco-enzyme treatments (p<0.05). This intermediate concentration permitted ingestion and disruption of gut pH, digestive enzymes, and neural pathways, whereas higher concentrations provoked irritation and avoidance, reducing toxin uptake. Conclusion: Eco-enzyme at 25% provides optimal larvicidal activity, highlighting its potential as a biodegradable, low-cost alternative. Its application could support integrated pest management, lowering environmental residues, and promoting sustainable agriculture. Future work should include field validation, non-target assessments, and formulation strategies to enhance stability and scalability.
Effectivity of Calcium, Phosphate and Vitamin D in Dental Caries Prevention Pratiwi Soesilawati; Yasmin Firdausi Rezkika; Naqiya Ayunnisa; Maulida Putri Syarifina; Salsabilavi Soffarina; Imanuel Jeremias Laturiuw; Padma Cahyaning Pertiwi
Denta Journal Kedokteran Gigi Vol 17 No 2 (2023): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/denta.v17i2.4

Abstract

Background: Micronutrient elements are vitamins and minerals that are required in small amounts but play important roles in our body. Insufficient micronutrients can affect health including dental health. Objective: Analyzing effectiveness of micronutrients (calcium, phosphorus, and vitamin D) in preventing dental caries. Literature study: This literature study was conducted from Google Scholar, Science Direct, and Pubmed (MEDLINE).  The strategy used in literature collection is in the form of writing keywords that are used to find articles to be reviewed with a publication period between 2012 - 2020.  Discussion: Dental caries is a demineralization of an inorganic part of the tooth due to a multifactorial etiology and occurs when demineralization exceeds remineralization. The calcium and phosphorus ions level in the saliva could affect the balance between demineralization and remineralization of enamel and its deficiency can strongly influence morphology of the tooth. It has been found that calcium level significantly influences hard dental tissues defense mechanisms. On the other side, vitamin D also plays an important role to form enamel and dentin in the tooth development process because of its receptor in ameloblasts and odontoblasts. Deficiency of vitamin D can cause enamel hypoplasia which is a significant factor for early childhood caries. Conclusions: Calcium, phosphorus, and vitamin D significantly affecting the demineralization and remineralization process. The optimum concentration and level of calcium, phosphorus, and vitamin D in the body effectively increase the tooth defense mechanism and reduce the dental caries.
Co-Authors Agatha Sherly Ivana Dewi Aurelia Graceilla Putri Nindyatama Imanuel Jeremias Laturiuw Maulida Putri Syarifina Moses Amadeus Soetanto Naqiya Ayunnisa Padma Cahyaning Pertiwi Rafael Gerrard Salsabilavi Soffarina Yasmin Firdausi Rezkika Yoakim Leok Taboy