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All Journal Jurnal Kesehatan Gigi
Lestari Vidyahayati, Indah
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Dentistry

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Antibacterial Activity Of Toothpaste Formulation Containing Spirulina platensis Extract Against Streptococcus mutans Dharma Canti, Distca Putri; Lestari Vidyahayati, Indah; Natania Renata Purnomo, Brigitta
Jurnal Kesehatan Gigi Vol. 11 No. 1 (2024): juni 2024
Publisher : Jurusan Kesehatan Gigi, Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jkg.v11i1.11296

Abstract

Background: The main bacteria involved in the formation of dental caries is Streptococcus mutans (S. mutans). One approach to reduce the growth of S. mutans bacterial colonies is by brushing teeth with toothpaste containing antibacterial agent. The commonly used antibacterial agent is chlorhexidine, which is the gold standard chemical in reducing the colonization of S. mutans bacteria and other oral biofilms. However, over time, it causes various side effects, thus necessitating the search for alternative natural ingredients. One of  potential ingredient with antibacterial agent is the Spirulina platensis, which contains secondary metabolites known to inhibit the growth of pathogenic bacteria. Thus, Spirulina platensis can be added to toothpaste as an antibacterial agent. Method: This study employed an experimental laboratory design with a post-test-only control group design. The study consisted of 7 treatments with 5 replications. Antibacterial activity was tested using the agar diffusion method by observing the average diameter of the inhibition zone formed. Data were analyzed using parametric one-way ANOVA. Discussion and Results: In this study, toothpaste with Spirulina platensis extract at concentrations of 0.25%, 0.5%, 1%, 2%, and 3% can inhibited the growth of S. mutans with average inhibition zone diameters of 20.4 mm, 20.8 mm, 21.8 mm, 22.3 mm, and 22.7 mm. The one-way ANOVA parametric test results showed a value of p<0.05, indicating a significant difference in the antibacterial activity of S. mutans in all treatment groups. Conclusion: Toothpaste formulations with Spirulina platensis extract have antibacterial activity against S. mutans bacteria.
The Effectiveness Of Rinsing With 10% Forest Honey Solution on Plaque Accumulation in Fixed Orthodontic Patients Husna, Salsabila Aliyah; Dinar, Nushita; Lestari Vidyahayati, Indah
Jurnal Kesehatan Gigi Vol. 12 No. 1 (2025): December 2025
Publisher : Jurusan Kesehatan Gigi, Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jkg.v12i1.12146

Abstract

Fixed orthodontic appliances to correct malocclusion and maintain periodontal tissues have complex designs and appliance shapes that can accelerate plaque formation. Preventive efforts to maintain oral hygiene status during treatment of fixed orthodontic devices can be done by using alternative mouthwashes made from natural ingredients, one of them is forest honey. This study aimed to determine the effectiveness of rinsing with 10% forest honey solution on reducing plaque accumulation in fixed orthodontic appliance patients. Type of research was Quasi Experiment with Pre-Post Test with Control Group Design. A sample of 40 people was divided into a control group rinsing with distilled water and a treatment group rinsing with 10% forest honey solution for 4, 6, and 8 days. Assessment of plaque accumulation using the Orthodontic Plaque Index (OPI). Data test results were normally distributed and homogen (p>0.05). One Way Anova test showed a significant difference between OPI before and after in the control group and treatment group (p<0,05). Post Hoc LSD results showed insignificant values ​​(p>0.05) in group K (aquadest) and group P1 (4 days), while in group P2 (6 days) and P3 (8 days) rinsing 10% forest honey solution showed significant values ​​(p<0.05). Rinsing with 10% forest honey solution for 6 days and 8 days is effective in reducing plaque accumulation in fixed orthodontic appliance patients.
Co-Authors Dharma Canti, Distca Putri Husna, Salsabila Aliyah Natania Renata Purnomo, Brigitta Nushita Dinar