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Effendi , Alvira Noer
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Immunology & microbiology

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Shoot Induction of Pandanus Tectorius through Shoot Culture in WPM Medium Supplemented With BAP And NAA Matondang, Ikhsan; Effendi , Alvira Noer
Journal of Tropical Biodiversity Vol 5 No 1 (2024): December 2024
Publisher : Universitas Nasional Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59689/bio.v5i1.245

Abstract

The aim of this research is to determine the best shoot induction medium for Pandanus tectorius, a coastal plant valued for its ecological and economic benefits. Coastal degradation and land conversion have reduced its population, necessitating propagation efforts. In vitro culture was explored as a solution to address the scarcity of Pandanus tectorius. Woody Plant Medium (WPM) was prepared by combining 100 mL stock solution, 30 g sugar, and distilled water to 1 L, supplemented with 1 g activated charcoal, 0.1 g ascorbic acid, and plant growth regulators at varying concentrations. The pH was adjusted to 5.6–5.8, and agar was added before sterilization at 121 °C for 20 minutes. Explants consisting of 1 cm-long shoots were sterilized and cultured in bottles, with observations conducted over 8 weeks. Results showed that the best shoot induction was achieved using WPM supplemented with 5x10⁻⁶ M BAP and 5x10⁻⁶ M NAA, producing 1 to 3 shoots per explant. Data analysis using a factorial completely randomized design confirmed significant differences among treatments. This study demonstrates the effectiveness of in vitro propagation in restoring Pandanus tectorius populations and supports sustainable coastal management.
Co-Authors Ikhsan Matondang