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All Journal Indonesian Journal of Jamu
Zalukhu, Aurentinus
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Medicine & Pharmacology

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Antioxidant Capacity, Total Phenolic, and Flavonoid Content from Java Tea (Orthosiphon aristatus) Extracts Arif, Zulhan; Zalukhu, Aurentinus; Karomah, Alfi Hudatul; Rafi, Mohamad
Jurnal Jamu Indonesia Vol. 7 No. 3 (2022): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v7i3.268

Abstract

Orthosiphon aristatus, known as kumis kucing in Indonesia, has some biological activity, i.e., as an anti-inflammatory, fever, rheumatism, diabetes, and kidney stone. This plant is also known for being rich in phenolic compounds such as flavonoids. In this study, we would like to compare the antioxidant activity, total phenolics, and flavonoid content from the stems and leaves of Orthosiphon aristatus extracted with water and ethanol by maceration. The yield stem and leaves of the water extract are 12.98-31.28%, and ethanol extract 3.58 - 9.82% (dry basis). The stem and leaves water extract contains a phenolic of 7.82-26.43 (mg GAE/g) and ethanol extract of 2.27-7.84 (mg GAE/g). Water extract Orthosiphon aristatus flavonoid content is 1.23-2.86 (mg EK/g), and ethanol extract is 1.09-3.95 (mg EK/g). The antioxidant activity of ethanol (IC50), extract from leaves was 123.91 (µg/mL) and from stems was 125.50 (µg/mL), while IC50 of water extract from leaves was 114.70 (µg/mL) and from stems was 125.03 (µg/mL). The correlation test result of active compounds using linear regression is phenolic compounds with antioxidants stronger than flavonoids. The qualitative thin-layer chromatography fingerprint analysis showed that ethanol and water extract contained natural sinensetin compounds.
Co-Authors Karomah, Alfi Hudatul M. Rafi