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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology
SOFIYANTO, M. EDY
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

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SACCHARIFICATION OF CORNCOB USING CELLULOLYTIC BACTERIA FOR BIOETHANOL PRODUCTION SUNARTI, TITI CANDRA; MERYANDINI, ANJA; SOFIYANTO, M. EDY; RICHANA, NUR
BIOTROPIA Vol. 17 No. 2 (2010)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8621.122 KB) | DOI: 10.11598/btb.2010.17.2.80

Abstract

The use of cellulose degrading enzyme (cellulases) for hydrolysis of lignocellulosic material is a part of bioethanol production process.  In this experiment,  delignified corncob,  its cellulose fraction and alpha cellulose were used as substrates to produce fermentable sugar by using three local isolates of celluloytic bacteria (C5-1, C4-4, C11-1 and Cmix ; mixed cultures of three isolates), and Saccharomyces cereviseae to produce ethanol. The results showed that all isolates of cellulolytic bacteria can grow on cellulose fraction better than on  delignified corncob, and alpha cellulose.  The highest hydrolytic activity produced from cellulose fraction was by isolate C4-4, which liberated 3.50 g/l of total sugar.  Ethanol can be produced by mixed culture of bacteria and yeast, but because of competitive growth, the fermentation only produced 0.39-0.47 g/l of ethanol. Keywords:  Celluloses, delignified corncob
Co-Authors Anja Meryandini Nur Richana Titi Candra Sunarti