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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology
CHAI, MAK
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

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PLOJDY ANALYSIS AND DNA CONTENT OF MUTANT BANAN "PISANG BERANGAN" USING FLOW CYTOMETRY OTHMAN, ROFINA YASMIN; SHADIA, ABDELGADIR RAYIS; CHAI, MAK
BIOTROPIA No. 19 (2002)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2002.0.19.228

Abstract

Mutagens cause random changes in the nuclear DNA or cytoplasmic organelles, resulting in gene, chromosomal or genomic mutations and hence, create variability. In this study, flow cytometry (FCM) was used to determine ploidy levels and DNA content in gamma-irradiated variants of mutated Pisang Berangan (cv. Intan, AAA) - a local banana genotype. Induced variants such as short plant stature (stunted growth), late flowering plants (late maturity) and abnormalities in bunch characters were selected to study possible changes at the DNA level. The study showed that DNA content of mutated plants differed from non-irradiated control and that irradiation had the most effect at high doses (40 and 60 Gy). The increase of DNA content in 20 Gy and 30 Gy treated plants was not more than that of the control plants. The values of genomic DNA content of gamma-irradiation variants decreased as the dose of irradiation increased from 20 to 60 Gy, indicating that the high dose of gamma-irradiation had a significant effect on the genome of the plants. The analysis further showed that phenotypic variation due to mutagenesis was reflected in the DNA content of the plants. The results also showed that ploidy levels were not affected by gamma-irradiation even at high doses. Keywords: Musa spp./mutation breeding/ flow cytometry/  ploidy level/ DNA content
MORPHOLOGICAL CHARACTERIZATION OF MALAYSIAN WILD BANANA MUSA ACUMINATA JAVED, MUHAMMAD ASIF; CHAI, MAK; OTHMAN, ROFINA YASMIN
BIOTROPIA No. 18 (2002)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (253.366 KB) | DOI: 10.11598/btb.2002.0.18.170

Abstract

Fourteen populations of Musa acuminata  ranging from populations in  the lowlands of northern (ssp. siamea)  to central Malaysian region  (ssp. malaccensis) and highland banana  (ssp. truncata) were characterized based on chromosome number and 46 morphological characters. A large amount of variation was observed within the populations. However, only highland bananas appeared morphologically distinct. Lowland populations both from northern and central Malaysia were found to be overlapping and no distinguishing pattern was observed. The morphological characters found variable within these populations were related to developmental changes and mutations. The results obtained in this study were not revolutionary. However, the survey of a large number of characters treated with multivariate techniques further sharpened the existing groupings of the Musa acuminata subspecies. Key words: Musaceae/ Malaysia / ssp. malaccensis I ssp. truncata I ssp. siamea
Co-Authors JAVED, MUHAMMAD ASIF OTHMAN, ROFINA YASMIN SHADIA, ABDELGADIR RAYIS