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All Journal JOURNAL OF CHEMICAL NATURAL RESOURCES (JCNaR)
Sembiring, Helmina
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Biochemistry, Genetics & Molecular Biology Chemistry Materials Science & Nanotechnology

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Antibacterial Activities of Rough Lemon (Citrus jambhiri Lush) Rind Essential Oil Sembiring, Helmina
Journal of Chemical Natural Resources Vol. 1 No. 1 (2019): Journal of Chemical Natural Resources
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (710.467 KB) | DOI: 10.32734/jcnar.v1i1.830

Abstract

The isolation of rough lemon (Citrus jambhiri Lush) rind essential oil has been done by using hydrodistillation method and paper disk diffusion test for antibacterial activity test. 2.3 gram (1.51%) of essential oil was obtained from 450 gram of dried rough lemon rind powder. Based on the analysis using GC-MS, the essential oil from rough lemon rind contains 5 chemical compounds, but only 4 of them can be interpreted. They are Limonene (71.88%), 1,4-cyclohexadiene (13.93%), β-Pinene (10.57%) and β-Ocimene (2.37%). Antibacterial activity was tested to Bacillus cereus and Escherichia coli at 40% concentration (v/v) with 12.7 mm and 12.9 mm of clear zones respectively.
Isolation and Identification of Flavonoid Compound from the Rambutan Stem Bark (Nephelium lappaceum L.) Sembiring, Helmina; Jelisa, Mesy
Journal of Chemical Natural Resources Vol. 6 No. 1 (2024): Journal of Chemical Natural Resources
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jcnar.v6i1.16207

Abstract

Flavonoids derived from the stem barks of Rambutan (Nephelium Lappaceum L.) have been extracted. The extraction of Rambutan stem bark was performed by maceration using a methanol solvent. The concentrated methanol extract was diluted in ethyl acetate until the solution became devoid of all positive and flavonoid compounds. The highly concentrated ethyl acetate extract was subsequently dissolved using methanol and separated into different parts using an n-hexane. The methanol content was determined using thin-layer chromatography and separated using column chromatography using eluent chloroform: methanol in various ratios (90:10, 80:20, 70:30) v/v. The chemicals were purified using preparative thin-layer chromatography (TLC) and yielded 7.9 mg of amorphous solids with an Rf value of 0.25. The eluent was a chloroform and ethyl acetate mixture in a 50:50 volume-to-volume ratio. The substance was analyzed using Spectrophotometer Ultraviolet Visible (UV-Vis), Fourier Transform Infra Red Spectrophotometer (FT-IR), and Proton Nuclear Magnetic Resonancy Spectrophotometer (1H-NMR). The analysis identified the chemical as a flavonoid of the flavonol.
Co-Authors Jelisa, Mesy