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All Journal JOURNAL OF CHEMICAL NATURAL RESOURCES (JCNaR)
Purba, Yuni Romasni
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Biochemistry, Genetics & Molecular Biology Chemistry Materials Science & Nanotechnology

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Isolation and Identification of Phenolic Compounds from Ebony Plant Leaves (Diospyros celebica Bakh.) Purba, Yuni Romasni; Sembiring, Helmina Br.
Journal of Chemical Natural Resources Vol. 4 No. 2 (2022): Journal of Chemical Natural Resources
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jcnar.v4i2.11972

Abstract

Isolation of phenolic compounds from Ebony plant leaves (Diospyros celebica Bakh .) was done by maceration technique with methanol solvent. The concentrated extract of methanol dissolved with distilled water and partition was extracted with ethyl acetate. The concentrated ethyl acetate extract dissolved with methanol solvent and partitioned with n-hexane. Then Thin layer chromatography was analyzed before column chromatography, and the eluent suitable for separation is chloroform: methanol 90:10, 80:20, 70:30,60:40,50:50 (v/v) as mobile phase. The compounds were purified with a thin layer preparative and crystallization yielding yellow paste weighing 7.5 mg with Rf = 0.45. The test purity of compounds is done by thin layer  chromatography showing a single spot with eluent chloroform: ethyl acetate (50:50) v/v and n-hexane: ethyl acetate ( 80:20) v/v. The following compound was further indicated by Ultraviolet-Visible Spectrophotometer (UV-Vis), Fourier Transform Infra Red Spectrophotometer (FT-IR), and Proton Nuclear Magnetic Resonancy Spectrophotometer (1H-NMR) was estimated as a phenolic methyl gallate, phenolic acid.
Co-Authors Sembiring, Helmina Br.