Article Per Year (5 Year)
p-Index From 2020 - 2025
0
P-Index
This Author published in this journals
All Journal Annales Bogorienses
Malle, Domininggus
Unknown Affiliation

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
Rapid Purification and Partial Characterization of an Extracellular Enantioselective Lipase from Aspergillus niger Malle, Domininggus; Garingan, Gina; Peralta, Milagros; Revilleza, Maria Jamela
Annales Bogorienses Vol. 12 No. 1 (2008): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Lipases [EC. 3. 1.1 .3] are capable of hydro lyzing ester bonds of triglycerides and have received much attention from protein researchers after these enzymes have shown potential applications such us, in the manufacture of commercially important crural drugs due to their enantioselectivity properties. A novel extracellular lipase produced by Aspergillus niger was purified and partially characterized. Using the culture medium as crude extract, the Lipase was purified to 25 .7 folds after Ultrafiltration and DEAE ion exchange chromatography. The enzyme was characterized to have optimum activity at neutral pH and at 30-35°C, while its kinetic parameters, Vmax and Km, were determined to be 1.80 U/mL/min and 0.2 mL, respectively. SDS-PAGE analysis revealed the presence of at least two bands (±87 and ±72 kDa), while native PAGE showed a single band. The two bands could represent subunits in a complex or isoforms of the enzyme. The use of the enzyme in the hydrolysis of a racemic mixture of 2-arylpropionic butyl ester analyzed after HPLC demonstrated an apparent predominant enantioselectivity for the S (+) enantiomer. Thus, this lipase is a promising enzyme for the chiral drug preparation of single enantiomer of 2-arylpropionic acid (ibuprofen).
Co-Authors Garingan, Gina Peralta, Milagros Revilleza, Maria Jamela