The advancement of digital technology has significantly driven innovation in health applications, offering users practical tools to monitor their physical and emotional well-being. Among these, Flo: Period & Pregnancy Tracker stands out as a popular application designed to help women track their reproductive cycles, ovulation, and associated hormonal symptoms. This study aims to evaluate the user experience of new users of the Flo application across two age groups: 12–25 years and 26–45 years, to understand their perceptions of comfort and ease of use, employing a quantitative approach with the User Experience Questionnaire (UEQ). Analysis results indicate that both age groups generally provided positive assessments of the application. The Stimulation and Efficiency aspects received the highest scores, while Novelty was the lowest-scoring aspect. Further analysis revealed that the 12– 25 year age group tended to prioritize hedonic qualities (such as Stimulation and Attractiveness), whereas the 26– 45 year age group valued pragmatic qualities (such as Efficiency and Perspicuity) more in their initial app usage experience. These findings underscore the importance of UI/UX design that adapts to the differing needs and expectations of users across age segments for overall experience improvement. It is important to note that the imbalance in the number of respondents between age groups is a limitation of this study, which may affect the validity of peer-to-peer comparisons and the generalizability of results due to constraints in time and primary respondent data availability.