Graphical Abstract Highlight Research First to assess KAP on post-harvest fish loss among fisherfolks, vendors, and processors in the Philippines. Processors had higher knowledge; fisherfolks showed better attitudes and practices. Daily fish output and gender significantly influenced knowledge and practices. Recommends targeted training based on roles and gender to reduce fish loss. Abstract Fisheries postharvest losses (PHLs) lead to significant economic and nutritional impacts, particularly in developing countries like the Philippines. Despite the availability of post-harvest facilities, losses remain high due to inadequate handling practices. This study aimed to evaluate the knowledge, attitudes, and practices (KAP) of fisherfolk, vendors, and processors at the Lucena Fish Port Complex and to determine how socio-demographic factors influence these practices, in order to guide interventions that can reduce post-harvest fisheries loss. The KAP related to PHLs was assessed among fisherfolks, vendors, and processors at the Lucena Fish Port Complex (LFPC) and sociodemographic factors influencing KAP was determined. A cross-sectional survey was conducted among 390 respondents using structured questionnaires. Data were analyzed using descriptive statistics, chi-square tests for association, Kruskal-Wallis H test with Dwass-Steel-Critchlow-Fligner (DSCF) post-hoc tests for group comparisons, and multiple linear regression to identify predictors of KAP scores. Significant group differences were found among groups. Processors had greater knowledge of PHLs, fisherfolk showed stronger attitudes toward loss reduction, while vendors displayed inconsistent practices in storage, icing, and sorting. Daily output predicted higher knowledge and practice scores, and males were more likely to follow recommended handling methods. The results suggest that targeted training programs considering stakeholder roles and gender dynamics are essential to reduce PHLs. Recommended interventions include capacity-building on hygienic handling and proper icing, improved access to affordable cold chain facilities, gender-responsive training for women vendors and processors, and awareness campaigns on the economic benefits of reducing PHLs. Future research should also investigate the extent and specific types of PHLs experienced by different stakeholders, as well as assess the effectiveness and long-term impact of targeted capacity-building interventions on improving handling practices and reducing PHLs.