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All Journal Biocelebes Journal of Tropical Biodiversity and Biotechnology BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Jurnal Biologi Tropis Advances in Tropical Biodiversity and Environmental Sciences
Mustafid Rasyiid
Faculty Of Biology, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Immunology & microbiology Veterinary

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Immunomodulatory and Antioxidant Activity of Green Grass Jelly Leaf Extract (Cyclea barbata Miers.) In Vitro Rendi Mahadi; Mustafid Rasyiid; Krisnanda Surya Dharma; Lindia Anggraini; Rahma Nurdiyanti; Tri Rini Nuringtyas
Journal of Tropical Biodiversity and Biotechnology Vol 3, No 3 (2018): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.33441

Abstract

Green grass jelly (Cyclea barbata Miers.) is known for its benefit to human health especially in supporting body’s immune system and wellness. This research aimed to determine immunomodulatory and antioxidant activity of green grass jelly leaf extracts in vitro. Old leaves were collected as sample then dried and ground to powder. The extraction was done with sohxletation using three different solvents, chloroform, ethyl acetate, and ethanol. The immunomodulatory activity was evaluated by treating the crude extracts at concentrations of 50, 100, and 500 mg/mL on macrophages of rat in vitro. Macrophage cells separated form peritoneal fluid used RPMI medium. Phagocytosis activity and phagocytosis capacity of macrophages were performed in vitro using latex beads that suspended in phosphate buffered saline (PBS). The antioxidant activity was measured by spectrophotometry technique with 1,1-diphenyl-2-picrylhydrazyl (DPPH) solution. All treatments were done three replicates. Detection of the bioactive groups of the extracts was done by Thin Layer Chromatography (TLC). The results showed that ethyl acetate extract has the highest phagocytosis activity followed by chloroform extract and ethanol extract, respectively. Optimum concentration was reached at 100 mg/mL of ethyl acetate extract. The ethyl acetate extract was also the highest antioxidant activity index 7.7 followed by both extracts of chloroform and ethanol similar index value of 6.25 and 6.3, respectively. The ethyl acetate extract has a high immunomodulatory activity and antioxidant activity which contained phenolics, flavonoids, tannins, and terpenoids.
Immunomodulatory and Antioxidant Activity of Green Grass Jelly Leaf Extract (Cyclea barbata Miers.) In Vitro Mustafid Rasyiid; Rendi Mahadi; Krisnanda Surya Dharma; Lindia Anggraini; Rahma Nurdiyanti; Tri Rini Nuringtyas
Advances in Tropical Biodiversity and Environmental Sciences Vol 2 No 1 (2018): ATBES
Publisher : Institute for Research and Community Services Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (432.378 KB) | DOI: 10.24843/ATBES.2018.v02.i01.p03

Abstract

Green grass jelly (Cyclea barbata Miers.) is known for its benefit to human health especially in supporting body’s immune system and wellness. This research aimed to determine immunomodulatory and antioxidant activity of green grass jelly leaf extracts in vitro. Old leaves were collected as sample then dried and ground to powder. The extraction was done with sohxletation using three different solvents, chloroform, ethyl acetate, and ethanol. The immunomodulatory activity was evaluated by treating the crude extracts at concentrations of 50, 100, and 500 mg/mL on macrophages of rat in vitro. The treated macrophage was then challenged for their phagocytic activity to latex beads. The antioxidant activity was done using 1,1-diphenil-2-picrilhydrazil (DPPH) with spectrophotometry technique. All treatments were done with three replicates. Detection of the bioactive groups of the extracts was done by Thin Layer Chromatography (TLC). The results showed that ethyl acetate extract has the highest phagocytic activity followed with chloroform extract and ethanol extract, respectively. Optimum concentration was reached at 100 mg/mL of ethyl acetat extract. The ethyl acetate extract was also the extract with the highest antioxidant activity index 7.7 followed by both extracts of chloroform and ethanol with similar index value of 6.25 and 6.3, respectively. The ethyl acetate extract contained phenolics, flavonoids, tannins, and terpenoids.
POPULASI & POLA DISTRIBUSI Buchanania arborescens (Blume) Blume (Anacardiaceae) PADA TANAH ULTRA- MAFIK DESA PODI, KECAMATAN TOJO, KABUPATEN TOJO UNA-UNA, SULAWESI TENGAH Pitopang, Ramadanil; Syam, Nurfadilah; Rasyiid, Mustafid
Biocelebes Vol. 18 No. 1 (2024)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v18i1.17197

Abstract

This study aims to determine the distribution pattern and population density of Buchanania arborescens (Blume) Blume in Ultramafic Soil, Podi Village, Tojo District, Tojo Una-Una District, Central Sulawesi. Observations were made using the purposive sampling method by establishing five (5) transects 50x10 m in size which there were subplots with size 10x10 m to observe tree (DBH >10 cm), 5x5 m for the sapling and 2x2 m for seedling with a distance of 20 m between transects. The distribution pattern of Buchanania arborescens at the study site was determined by calculating the morbidity index with the results of calculations on seedling phase Id (0.04), sapling Id (0.05) and tree Id (-1.00). The highest density of Buchanania arborescens is in the seedling phase with a value of 200 individuals/Ha, the sapling phase is 176 individuals/Ha and the lowest value is in the tree phase with a total density of 40 individuals/Ha. The results showed that the distribution pattern of Buchanania arborescens for the seedling and sapling phases were clumped, while the tree phase was uniform. The density of Buchanania arborescens was rare density category.
MELISSOPALYNOLOGICAL ANALYSIS OF HONEY FROM DOLAGO VILLAGE, CENTRAL SULAWESI Rasyiid, Mustafid; Iqbal, Moh.; Paserang, Asri Pirade; Budiono, Yudhistira Kristinov; Nugraha, Muh. Ardian Satrio; Wiguna, Triadhy
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 11 No. 2 (2025): Biolink February
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v11i2.13402

Abstract

One thing that honeybees create that is very beneficial to human health is honey. Consequently, honey is frequently faked. With melissopalynology analysis, honey quality can be ascertained. A technique for analyzing pollen in honey to ascertain its botanical and geographic origin is called melissopalynology analysis.This study uses melissopalynology to analyze the quality and pollen composition of honey produced by Apis cerana bees in Dolago Village, Central Sulawesi. The research consists of five stages: collecting honey, extracting pollen from it using a modified acetolysis method, identifying pollen, classifying honey, and last, absolute pollen count. The analysis identified 11 plant species contributing to honey production, with Cocos nucifera as the predominant pollen (70%). Classified as unifloral honey with Group IV pollen richness, this research confirms the high quality and unique floral origin of Dolago honey, emphasizing its potential for local economic development.
Anatomy of Sarcotheca celebica’s vegetative organs growing in Morowali on Ultramafic and Limestone Soils Harso, Wahyu; Rasyiid, Mustafid; Ramadanil, Ramadanil; Umrah, Umrah; Iqbal, Moh.
Jurnal Biologi Tropis Vol. 25 No. 2 (2025): April-Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i2.8626

Abstract

Sarcotheca celebica is an endemic Sulawesi plant capable of growing in ultramafic and calcareous soils. The anatomical structure of its vegetative organs plays a role in adaptation to extreme environments. This study aimed to compare the anatomy of leaves, stems and roots of S. celebica in both soil types. Microscopic preparations of leaves, stems and roots were made with the Free Hand Section method, documented using Optilab Viewer 2.2, and measured with Image Raster 3. Statistical analysis was conducted with the T-test. The results showed anatomical variations in response to soil conditions. S. celebica plants in calcareous soil had longer and wider stomata, but with lower density than those in ultramafic soils. In addition, leaf and cuticle thickness were higher in S. celebica plants on calcareous soils. However, the peridemis/floem ratio in stems and peridermis/endodermis in roots did not show significant differences. Overall, S. celebica leaves were more responsive to soil differences than stems and roots. This study is important for understanding the mechanisms of plant adaptation to extreme soil conditions and the implications for the sustainability of the species in its natural habitat.
POLLEN MORPHOLOGICAL DIVERSITY OF ASTERACEAE SPECIES IN THE TADULAKO UNIVERSITY CAMPUS AREA Budiono, Yudhistira Kristinov; Rasyiid, Mustafid; Musa, Khairul; Iqbal, Moh.
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 12 No. 1 (2025): Biolink August
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v12i1.14679

Abstract

This study aims to explore the morpho-anatomical diversity of pollen from various plant species belonging to the Asteraceae family within the Tadulako University area. Flower samples were collected using an exploration method and prepared for palynological analysis using a modified acetolysis technique. Six Asteraceae species were identified: Tridax procumbens L., Cyanthillium cinereum (L.) H.Rob., Synedrella nodiflora (L.) Gaertn., Eleutheranthera ruderalis (Sw.) Sch.Bip., Porophyllum ruderale (Jacq.) Cass., and Chromolaena odorata (L.) R.M.King & H.Rob. The study revealed significant differences in pollen morphology, particularly in shape (spheroidal), aperture number (3–4 colporates), ornamentation (echinate vs. lophate), and size. Pollen size ranged from 21.29 µm to 35.66 µm (equatorial diameter) and wall thickness ranged from 2.16 µm to 4.82 µm. These variations serve as reliable taxonomic markers for distinguishing species at both genus and species levels. This research provides valuable insights into the biodiversity and evolutionary adaptations of the Asteraceae family and supports the use of palynological data in systematic botany.
Co-Authors Asri Pirade Paserang Budiono, Yudhistira Kristinov Krisnanda Surya Dharma Krisnanda Surya Dharma Lindia Anggraini Lindia Anggraini Moh. Iqbal, Moh. Musa, Khairul Nugraha, Muh. Ardian Satrio Rahma Nurdiyanti Rahma Nurdiyanti Ramadanil Pitopang Ramadanil, Ramadanil Rendi Mahadi Rendi Mahadi Syam, Nurfadilah Tri Rini Nuringtyas Umrah Umrah Wahyu Harso Wiguna, Triadhy