Ni Ketut Suwiti
Laboratorium Histologi Veteriner, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, 80234, Indonesia

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HISTOMORPHOMETRIC FEATURES DAN PERCENTAGE OF NEUTROPHILS AND BASOPHILS IN BALI CATTLE BASED ON CONVENTIONAL SYSTEM Angela Ivanka Benedicta; Ni Ketut Suwiti; Ni Nyoman Werdi Susari; Ni Luh Eka Setiasih; I Ketut Suatha; Anak Agung Sagung Kendran
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2024 Vol. 16 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i02.p24

Abstract

The Bali cattle rearing system in Bali is generally carried out using a conventional system. Conventional rearing is characterized by raising livestock on an area of land with food sources originating from the environment, so that the health of Bali cattle receives less attention. This maintenance method affects the body's resistance, one of the indicators is white blood cells. This study aims to determine the histology, morphometry and white blood cell count of neutrophils and basophils in Bali cattle based on conventional system. This research used samples in the form of blood from 30 Bali cattles, located in Bulian Village, Kubutambahan District, Buleleng Regency. Blood smears were fixed and stained using MDT IndoReagen®. Morphometric measurements of neutrophil and basophil white blood cells using the EP View application with 1000x magnification. Counting the number of neutrophils and basophils is done by counting from the edge of the field to the next edge, up to 100 leukocyte cells. The results showed that neutrophils have light pink, thin granular cytoplasm and have a segmented nucleus of 3-6 dark purple lobes with an average diameter of 5.79±0.57 µm. Basophils have purple granular cytoplasm and have a dark purple nucleus that is multi-segmented (polymorphonuclear) with an average diameter of 6.53±0.78 µm. The histological structure of neutrophils and basophils in Bali cattle based on conventional system is similar to neutrophils in other ruminants. The total number of neutrophil white blood cells was 14% and basophils was 8%. Further research needs to be done regarding health status by looking at other indicators such as the presence of white blood cells, eosinophils, lymphocytes and monocytes in Bali cattle based on conventional systems.
A STRUCTURE AND HISTOMORPHOMETRY OF BALI DUCK TESTES IN THE GROWER PHASE Ni Made Santi Rahayu Adiari; Ni Luh Eka Setiasih; I Putu Sampurna; Ni Ketut Suwiti; Ni Nyoman Werdi Susari; I Ketut Suatha
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2024 Vol. 16 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i02.p17

Abstract

Testes are male reproductive organs which are responsible for producing spermatozoa and steroid hormones. This study aims to determine the histology and histomorphometry structure of bali duck testes in the grower phase at the age of 12 weeks in one of the farms in Mengwi District, Badung Regency. The number of samples used in this research was 10 male bali ducks that were in good health. There are two examination methods carried out, namely the qualitative descriptive anatomical structure and histology examination method and the quantitative histomorphometry examination method of bali duck testicles which are analyzed using the One-Sample T-test with a confidence level of 95%. The results of this study show the anatomical structure of the bali duck testicles which are located in the abdominal cavity, between the kidneys and the lungs. The pair of testicles have the shape of a bean and are cream colored. The results of research on the histological structure of bali duck testes consist of seminiferous tubules, interstitial tissue, leydig cells, and tunica albuginea. The results of histomorphometry measurements of bali duck testes showed that the wall thickness of the seminiferous tubules was 23.6760 ± 1.4425 (µm), the number of seminiferous tubules was 421.1000 ± 56.1396 and the thickness of the tunica albuginea was 29.5000 ± 5.048 (µm). Considering the importance of knowledge about the structure of bali duck testicles, it is recommended for future researchers to examine the structure and histomorphometry of bali duck testicles at different ages in the growing phase.