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All Journal Buletin Veteriner Udayana
Luh Made Sudimartini
Laboratorium Farmakologi dan Farmasi Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia, 80234
Immunology & microbiology Public Health Veterinary

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IDENTIFICATION OF CHEMICAL COMPOUNDS ETHANOL EXTRACT LEAF MIMOSA THAT GROWS IN DENPASAR CITY Kadek Ayu Wiadnyani; Anak Dharmayudha; Luh Made Sudimartini; I Made Merdana
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2024 Vol. 16 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bvu.v16i1.58

Abstract

Indonesia is a tropical country rich in natural resources including medicinal plants, which have been used as traditional medicines for generations. Mimosa (Mimosa pudica L.) is a plant that has a lot of potential to be developed into raw materials for traditional medicine because it has many pharmacological activities that are beneficial in supporting health. This study aims to determine the active phytochemical compounds contained in the ethanol extract of Mimosa leaves. Mimosa leaves will be collected around the city of Denpasar, Bali. The sample will then be extracted using 70% ethanol solvent by maceration method. Phytochemical tests which include steroid/triterpenoid tests, flavonoid tests, alkaloid tests, phenolic tests, tannin tests, and saponin tests were carried out to identify the active phytochemical compounds in the samples. The data obtained were analyzed descriptively qualitatively. Based on testing of the phytochemical compounds of the 70% ethanol extract of mimosa leaves grown in the city of Denpasar, it was concluded that the extract contains triterpenoid/steroidal, alkaloids, flavonoids, phenolics, tannins, and saponins. This research is an early stage in detecting the content of secondary metabolites in mimosa. Further research is suggested to be carried out in testing the content of mimosa leaves, for example fractionation, which can later be used in practical applications in the field of veterinary medicine.
MICROBIAL CONTAMINATION TEST OF MIMOSA LEAVES SIMPLICIA Kezia Joana Limarta; Luh Made Sudimartini; Anak Dharmayudha
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2024 Vol. 16 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bvu.v16i1.59

Abstract

The Mimosa plant (Mimosa pudica L.) is a wild plant that can be easily found in Indonesia and all of its parts have been used in traditional medicine, especially the leaves. Seeing its pharmacological content and benefits, the leaves of the Mimosa plant have the potential to be used as a simplicia as raw material for herbal medicine. This study aims to determine microbial contamination of mimosa leaves simplicia according to the General Standard Parameters of Medicinal Plant Extracts of the Ministry of Health of Republic of Indonesia in 2000 and Regulation of the Head of Food and Drug Monitoring Agency of Republic of Indonesia Number 32 Year 2019 concerning Quality Requirements for Traditional Medicines. This research is a non-experimental observational research with a comparative descriptive analysis design. The microbial contamination level was assessed by Total Plate Count (TPC) test and Total Yeast and Mold Count (TYMC) test. The research data obtained is quantitative data which was analysed by counting the number of microbes that grew on Plate Count Agar (PCA) media and Potato Dextrose Agar (PDA) media after being incubated at the appropriate growth temperature. The Total Plate Number of the Mimosa leaves sample is 3,9x104 CFU/gram and the Total Yeast and Mold Count is 2,5x103 CFU/gram. The results showed that the mimosa leaf simplicia met the microbial contamination requirements for Total Plate Number (£5x107) and Total Yeast and Mold Count (£5x105). It can be concluded that the mimosa leaves simplicia sample can be processed into drug preparations. Further research on pathogen Escherichia coli, Enterobacteriaceae, Clostridia, Salmonella, and Shigella microbial contamination and other quality standardization tests are needed.
THE EFFECT OF NATURAL GUARD SUPPLEMENT IN BROILER DRINKING WATER ON THE HEALTH AND SAFETY OF THE LIVER, SPLEEN, AND KIDNEYS Millaniladzim Baadilla; Ida Bagus Komang Ardana; I Ketut Eli Supartika; I Made Merdana Merdana; Luh Made Sudimartini
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2025 Vol. 17 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i01.p18

Abstract

The broiler industry in Indonesia has grown rapidly since the 1970s and has become a primary source of animal protein. However, the health of vital organs such as the liver, spleen, and kidneys plays a crucial role in broiler productivity. Natural Guard (NG) is a feed supplement claimed to benefit these organs, but research on its safety remains limited. This study aimed to evaluate the effect of NG supplementation through drinking water on the health of the liver, spleen, and kidneys in broilers. A completely randomized design (CRD) was used, with four treatment groups: control without NG (P0) and NG supplementation at 1 g/L (P1), 2 g/L (P2), and 4 g/L (P3). Histopathological analysis was performed using Hematoxylin-Eosin (HE) staining, and data were analyzed using the Kruskal-Wallis test. The results showed no necrotic lesions in any group, while congestion and inflammatory cell infiltration were observed at mild to moderate levels without significant differences (P>0.05). This indicates that NG supplementation up to 4 g/L does not cause harmful pathological changes in the liver, spleen, and kidneys of broilers. In conclusion, NG is safe to use as a feed supplement for broilers. Further research is needed to assess its long-term effects and impact on broiler productivity.
Co-Authors Anak Dharmayudha Anak Dharmayudha I Ketut Eli Supartika I Made Merdana I Made Merdana I Made Merdana Merdana Ida Bagus Komang Ardana Kadek Ayu Wiadnyani Kezia Joana Limarta Millaniladzim Baadilla