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All Journal Buletin Veteriner Udayana
I Nengah Kerta Besung
Laboratorium Bakteriologi dan Mikologi Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana, Jl. PB. Sudirman Denpasar, Sanglah, Bali, Indonesia, 80234
Immunology & microbiology Public Health Veterinary

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YEAST POPULATION OF SACCHAROMYCES CEREVISIAE IN THE FECES OF YOUNG AND ADULT ETAWA CROSSBREED GOATS IN SUKAWATI DISTRICT, GIANYAR REGENCY Stephanie Levina; I Nengah Kerta Besung; I Gusti Ketut Suarjana
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2024 Vol. 16 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i3.p16

Abstract

Sacharomyces cerevisiae is a yeast that has great potential to increase the nutritional value of feed ingredients and increase the activity of rumen microbial performance, by utilizing oxygen so that rumen conditions are anaerobic, which makes cellulolytic bacteria develop well. This study aims to determine the population of S. cerevisiae yeast in young and adult etawa crossbreed goat feces. The samples used were 27 of etawa crossbreed goat feces from etawa crossbreed goat farms in Sukawati Sub-District, Gianyar Regency. There are three treatments given, namely etawa crossbreed goat feces from young goats aged 0-2 months, 3-5 months and adult goats aged over 6 months. This study used a Randomized Group Design by comparing the yeast population of S. cerevisiae in the feces of young and adult etawa crossbreed goats, which was done microscopically using a hemocytometer. The results showed that the average yeast content of S. cerevisiae in the feces of young etawa crossbreed goats aged 0-2 months was 25.56 x 104 ± 16,666.667 cells/ml, 3-5 months 42.22 x 104 ± 26,352.314 cells/ml, and adult goats aged 6 months to above 94.44 x 104 ± 30,459.445 cells/ml. There is a very significant difference, the burden of the yeast S. cerevisiae in the feces of adult Etawa crossbreed goats is higher compared to young goats. Further testing needs to be done on the type of yeast found in the feces and stomach of Etawa crossbreed goats.
PREVALENCE OF ESCHERICHIA COLI, COLIFORM AND NON-COLIFORM BACTERIA IN MUD BUFFALO IN RANGGAGATA AND SERAGE VILLAGES, SOUTHWEST PRAYA SUBDISTRICT, DISTRICT OF CENTRAL LOMBOK, WEST NUSA TENGGARA Erwin Satriawan; I Nengah Kerta Besung; I Gusti Ketut Suarjana
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2024 Vol. 16 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i3.p14

Abstract

Escherichia coli (E. coli) is a common bacterium that inhabits the intestines of humans and animals with hundreds of different strains, both harmful and harmless. One of the diseases that can be caused by E. coli is diarrhea. Diarrhea is a disease in the form of increased frequency of defecation, thinner stool consistency and increased intestinal peristalsis. This study aims to determine the number of E. coli, coliform and non-coliform bacteria in mud buffaloes in Ranggagata and Serage Villages, Southwest Praya Subdistrict, Central Lombok Regency, West Nusa Tenggara with samples used are fresh feces samples, a total of 14 samples. Sample cultivation was carried out on Eosin methyleneblue agar (EMBA) media then samples suspected of E. coli colonies were Gram stained, and biochemical tests on Triple Sugar Iron Agar (TSIA) media, Sulfide Indole Motility (SIM), Methyl red-Voges Proskauer (MR-VP) media, Simmons Citrate and catalase tests. The results showed the number of bacterial populations varied depending on the age of the buffalo with an average number of E. coli bacteria 162.143 ± 196.476, average coliform 272.000± 328.307 and average non-coliform 110.714 ± 201.855. Based on the results of the study it can be concluded that there is an increase in the number of E. coli, coliform and non-coliform as the age of buffalo increases, namely for E. coli is Y = 2.527+0.009x, coliform is Y = 2.525+0.05x and non-coliform bacteria is Y = 3.199+0.007x. Further tests need to be conducted related to other strains or species of E. coli, coliform and non-coliform bacteria in mud buffalo feces.
TOTAL BACTERIA, COLIFORMS AND NON-COLIFORMS IN THE MILK OF ETAWA CROSSBREED IN SINGAPADU KALER VILLAGE, SUKAWATI DISTRICT, GIANYAR REGENCY Katharina Diena Bhoki; I Nengah Kerta Besung; I Gusti Ketut Suarjana
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2024 Vol. 16 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i3.p28

Abstract

Etawa crossbreed goat's milk is a source of animal protein that is increasingly in demand by the public because it has very high nutritional value. Besides its nutritional value, milk is also a good medium for the growth of various types of microorganisms. This study aims to determine the total bacteria, coliforms and non-coliforms in Etawa crossbreed goat milk. The data taken is milk from the entire population of Etawa crossbreed goats that are currently lactating. This research is a type of observational research with a purposive sampling method. Counting total bacteria, coliforms and non-coliforms using the spread plate technique. The data obtained were analyzed descriptively by counting the number of bacteria in the culture media. The results showed that the total bacteria average was 3 x 10⁶ ± 1 x 10⁶ cfu/ml, coliforms were 2 x 10³ ± 1 x 10³ cfu/ml and non-coliforms were 2.2 x 10⁶ ± 2.5 x 10⁶ cfu/ml. Based on the research results, it can be concluded that the microbial contamination limit does not meet SNI. It is necessary to improve the cleanliness of cages and livestock so that bacterial contamination in milk can be reduced and further tests need to be carried out on the types of pathogenic and zoonotic bacteria present in the milk of Etawa crossbreed goats in Singapadu Kaler Village, Sukawati District, Gianyar Regency
Co-Authors Erwin Satriawan I Gusti Ketut Suarjana Katharina Diena Bhoki Stephanie Levina