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All Journal Buletin Veteriner Udayana
Wayan Bebas
Laboratorium Reproduksi Veteriner, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman, Sanglah, Denpasar, Bali; Indonesia, 80234
Immunology & microbiology Public Health Veterinary

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ADDITION OF VARIOUS CONCENTRATIONS OF SUGARCANE WATER TO EGG YOLK PHOSPHATE DILUENT ON THE QUALITY OF CEMANI CHICKEN SPERMATOZOA STORED FOR 48 HOURS Ni Wayan Ayu Sri Sedani; Wayan Bebas; I Wayan Sukernayasa
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p20

Abstract

Semen diluent is a solution that serves to maintain the quality of spermatozoa during storage and facilitate artificial insemination. The use of natural-based diluents is still rare, so it is necessary to develop diluents that utilize available natural resources. This study aimed to evaluate the effect of adding various concentrations of sugarcane water in egg yolk phosphate diluent on the quality of Cemani chicken semen stored for 48 hours at 5°C. This study used three cemani chickens with an age range of approximately seven months, in a healthy condition, and trained to collect sperm. The process of collecting chicken semen is done by massage method. This study used a completely randomized design with six treatments, namely the addition of sugarcane water as much as 0%, 5%, 10%, 15%, 20%, and 25%. Semen was stored for 48 hours at 5˚C. Parameters observed were motility, viability, and abnormality of spermatozoa. Each treatment was repeated four times. Data were analyzed using ANOVA test, followed by Ducan test if there were significant differences. The results showed that 20% egg yolk phosphate diluent with various concentrations of water had a significant effect (P<0.05) on motility, viability, and abnormality of cemani chicken semen. After the Ducan test, treatment T3 (phosphate buffer + 20% egg yolk + 15% cane water) produced the best semen quality, when compared to T0, T1, T2, T4, and T5, with a motility percentage of 62.50 ± 2.08%, viability 70.75 ± 2.75%, and abnormality 8.25 ± 0.96%. So, it can be concluded that the diluent formula that has the best results on the quality of spermatozoa stored for 48 hours is the treatment in phosphate buffer 20% egg yolk + 15% sugarcane water.
THE EXTENDED STORAGE OF PORK SEMEN DILUTED WITH PANYL FRUIT WATER EGG YOLK WITH THE ADDITION OF MORINGA LEAF EXTRACT I Kadek Agus Arsadana; Wayan Bebas; I Putu Sampurna
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2025 Vol. 17 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i02.p24

Abstract

Improving the genetic quality and population of pigs can be done through the technology of artificial insemination (AI), which requires semen storage under optimal conditions. This study aimed to evaluate the motility, viability, and abnormality of Landrace pig spermatozoa diluted using palm fruit water with the addition of 20% egg yolk and 15% Moringa leaf extract, and stored at 15°-20°C for 72 hours. This study used a completely randomized design method with four treatment groups based on the length of storage: 0 hours, 24 hours, 48 hours, and 72 hours. Fresh semen was collected from male Landrace pigs aged 1.5-2 years by massage method. Parameters measured were motility, viability, and abnormality of spermatozoa. Data were analyzed using ANOVA, followed by Games-Howell test if there were significant differences. The results showed that the length of storage had a significant effect on motility, viability, and abnormality of spermatozoa. After 72 hours of storage, sperm motility reached 40.83±1.17%, viability 53.50±1.38%, and abnormality 6.83±0.75%. The conclusion of this study is that the use of palm fruit water-based diluent with the addition of egg yolk and Moringa leaf extract still allows the storage of Landrace pig semen for 72 hours with acceptable quality. Further research is recommended to evaluate the potential of other additives to improve storability.
Co-Authors I Kadek Agus Arsadana I Putu Sampurna I Wayan Sukernayasa Ni Wayan Ayu Sri Sedani