I Wayan Sukernayasa
Laboratorium Reproduksi Veteriner, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman, Sanglah, Denpasar, Bali; Indonesia, 80234

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ADDITION OF VARIOUS CONCENTRATIONS OF SUGARCANE WATER TO EGG YOLK PHOSPHATE DILUENT ON THE QUALITY OF CEMANI CHICKEN SPERMATOZOA STORED FOR 48 HOURS Ni Wayan Ayu Sri Sedani; Wayan Bebas; I Wayan Sukernayasa
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p20

Abstract

Semen diluent is a solution that serves to maintain the quality of spermatozoa during storage and facilitate artificial insemination. The use of natural-based diluents is still rare, so it is necessary to develop diluents that utilize available natural resources. This study aimed to evaluate the effect of adding various concentrations of sugarcane water in egg yolk phosphate diluent on the quality of Cemani chicken semen stored for 48 hours at 5°C. This study used three cemani chickens with an age range of approximately seven months, in a healthy condition, and trained to collect sperm. The process of collecting chicken semen is done by massage method. This study used a completely randomized design with six treatments, namely the addition of sugarcane water as much as 0%, 5%, 10%, 15%, 20%, and 25%. Semen was stored for 48 hours at 5˚C. Parameters observed were motility, viability, and abnormality of spermatozoa. Each treatment was repeated four times. Data were analyzed using ANOVA test, followed by Ducan test if there were significant differences. The results showed that 20% egg yolk phosphate diluent with various concentrations of water had a significant effect (P<0.05) on motility, viability, and abnormality of cemani chicken semen. After the Ducan test, treatment T3 (phosphate buffer + 20% egg yolk + 15% cane water) produced the best semen quality, when compared to T0, T1, T2, T4, and T5, with a motility percentage of 62.50 ± 2.08%, viability 70.75 ± 2.75%, and abnormality 8.25 ± 0.96%. So, it can be concluded that the diluent formula that has the best results on the quality of spermatozoa stored for 48 hours is the treatment in phosphate buffer 20% egg yolk + 15% sugarcane water.
ASSOCIATION OF FOOT AND MOUTH DISEASE VACCINATION WITH FERTILITY PERFORMANCE OF BALI CATTLE ON FARMERS IN BADUNG DISTRICT Ni Luh Putu Ditha Rysdayeni; I Ketut Puja; I Wayan Sukernayasa
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2025 Vol. 17 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i02.p20

Abstract

Foot and mouth disease remains one of the most economically important infectious diseases in production animals. This disease can have many consequences, including reduced productivity, limited market access, and loss of livestock herds. Foot and Mouth Disease (FMD) vaccination is a crucial preventive measure to maintain livestock health, but its impact on the fertility performance of Bali cattle requires further investigation. This study aimed to analyze the association between FMD vaccination and the fertility performance of Bali cattle in Badung Regency. The study was conducted on 390 vaccinated Bali cattle, with fertility parameters observed including abortion, retained placenta, and repeat breeding rates. The results showed an abortion rate of 6.4% and a repeat breeding rate of 34.4%. Statistical analysis using the Test of Between-Subject Effects revealed a significance value of P<0.05, indicating a significant association between FMD vaccination and the fertility performance of Bali cattle. These findings provide important insights into reproductive management and highlight the need to consider vaccination aspects in cattle husbandry programs.