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Junita Hardini
PS Biologi FMIPA UNUD
Biochemistry, Genetics & Molecular Biology

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ISOLASI DAN IDENTIFIKASI JAMUR ENDOFIT PADA TANAMAN STROBERI (Fragaria × ananassa Duch. ‘Rosalinda’) DI DESA CANDIKUNING KABUPATEN TABANAN, BALI Meniwati; Anak Agung Darmadi; Junita Hardini
SIMBIOSIS Simbiosis Volume 12 No. 1 2024
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2024.v12.i01.p03

Abstract

Strawberries (Fragaria × ananassa Duch.) are subtropical plants well adapted to Indonesia’s highlands. Strawberries cultivated in Candikuning Village, Tabanan Regency, Bali are Fragaria × ananassa Duch. 'Rosalinda'. The fruit is widely used because it produces bioactive compounds. Bioactive compounds can also be obtained from endophytic fungi that live in host tissues. The study aimsed to identify the genus of endophytic fungi on the leaves and petioles of strawberries and to find out the benefits of the endophytic fungi found (literature review). This study used a descriptive method, which was carried out from December 2022 to February 2023. The stages in this study were the isolation of endophytic fungi, purification, and macroscopic and microscopic identification. There were 10 isolates of endophytic fungi isolated from leaves and petioles. Nine isolates were identified Aspergillus, Cercospora, Alternaria, Geotrichum, Penicillium, Cladosporium, Cylindrocladium, Cunninghamella, and Phytophthora. One isolate was not identified (mycelia sterile). These endophytic fungi are useful as biological controllers against plant pathogens, medicinal ingredients, and insecticides. Keywords: Candikuning, endophytic fungi, identification, isolation, strawberries
IDENTIFIKASI JAMUR ENDOFIT YANG BERASOSIASI DENGAN DAUN JERUK LIMAU Citrus x amblycarpa (Hassk.) OCHSE DI KEBUN KALPATARU, DENPASAR, BALI Riswara Nurfitri; Junita Hardini; I Ketut Muksin
SIMBIOSIS Simbiosis Volume 12 No. 1 2024
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2024.v12.i01.p11

Abstract

Endophytic fungi are fungi that live and associate in host plants and have the potential to produce secondary metabolites that are the same as their host. Utilization of endophytic fungi as a solution reduces the use of a lot of biomass to produce the desired secondary metabolites. This study aims to explore and identify endophytic fungi found on lime leaves obtained from Kalpataru plantation, Denpasar, Bali. Endophytic fungi were isolated from samples of young and mature leaves that were fresh, not wilted or yellow, and disease free. Fungal isolation was carried out by direct planting method on Potato Dextrose Agar (PDA) media at room temperature (27oC), and the incubation time was 3-7 days. Re-isolation was carried out to obtain pure cultures, then the endophytic fungi were identified by observing the morphological characters macroscopically and microscopically, then compared with identification books. The results obtained were eight isolates, and six isolates were identified belonging to the genera Fusarium, Colletotrichum, Aspergillus, Penicillium, and 2 isolates were not identified (sterile mycelia). Endophytic fungi found on young leaves, namely Aspergillus and Colletotrichum, on mature leaves, namely Fusarium, Aspergillus, and Penicillium. Keywords: endophytic fungi, lime, sterile mycelia, PDA
Co-Authors Anak Agung Darmadi I Ketut Muksin Meniwati Riswara Nurfitri