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Made Ria Defiani
PS Biologi FMIPA UNUD

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Journal : SIMBIOSIS

EKSTRAKSI DNA DAUN KUMIS KUCING (Orthosiphon stamineus Benth.) KERING Gusti Ayu Putu Intan Pandini; Made Pharmawati; Made Ria Defiani
SIMBIOSIS Simbiosis Volume 13 No. 1 2025
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2025.v13.i02.

Abstract

Orthosiphon stamineus Benth. is known as “kumis kucing” is one of the traditional medicinal plants widely used in Indonesia. Morphological identification of dried “kumis kucing” leaves as a herbal remedy is insufficient to prove the existence of counterfeiting practices, thus a more specific authentication method such as molecular genetic identification is required. However, before performing PCR, the selection of DNA extraction method with optimal quantity and quality is necessary. Therefore, this study aims to compare the effectiveness of the Doyle & Doyle method using CTAB, the Dellaporta method using SDS, and the Rogers & Bendich method in extracting DNA from dried “kumis kucing” leaves with the hope of providing information about the best DNA extraction method that can be used to identify the authenticity of “kumis kucing” leaves more accurately. The research method begins with sample preparation, DNA extraction, DNA quantity testing, electrophoresis, and visualization using a UV Transilluminator. Based on this research, the effective method for extracting DNA from dried “kumis kucing” leaves is the modified CTAB method (Doyle & Doyle, 1990), as it yields a high DNA concentration of 1508,33 ng/µL and has a faster processing time, taking 16 hours and 20 minutes. The visualization results show clear and thick DNA bands; however, the resulting DNA is not pure (A260/230: 1,01 and A260/280: 0,59).
PENINGKATAN PERTUMBUHAN VEGETATIF TANAMAN CABAI MERAH (Capsicum annuum L.) SETELAH APLIKASI EKOENZIM DAN PENYIRAMAN SECARA BERKALA Oktavia Cintya Firnawati; Martin Joni; Made Ria Defiani
SIMBIOSIS Simbiosis Volume 13 No. 1 2025
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2025.v13.i06.

Abstract

The growth of red chili plants (Capsicum annuum L.), is impacted by both environmental factors and nutrition availability. Ecoenzymes have the ability to lower global warming and meet plant nutrient needs. The research was conducted to analyze the vegetative growth of red chili plants after being given ecoenzyme treatment and gradual watering. Red chili seeds are sown for 4 weeks, then transferred to polybags. The research used a two-factor factorial randomized block design method, namely administering ecoenzymes with a concentration of 0%; 0,5%; 1%; 1,5% and watering gradually by giving water every day; twice a day; once in three days. Every combination of treatments was carried out three times. The study's findings demonstrated that, two weeks after seeding, an ecoenzyme concentration of 1,5% could provide the maximum germination capacity (90%) of any concentration. Age 12 Weeks After Planting in polybags, the maximum plant height (70,16 cm) at 0% ecoenzyme concentration and daily watering; the quantity of primary branches (10,78 branches) at 1,5% ecoenzyme concentration and daily watering. Age 16 Weeks After Planting, the largest leaf area (105,13cm2) measured using Image-J when applying an ecoenzyme concentration of 0% and watering every day (control); root length (28,33cm) in the treatment of 1,5% ecoenzyme concentration and watering every three days; The shoot dry weight was the greatest (3,29 g) and the root dry weight was the greatest (0,67 g) when the ecoenzyme concentration was 1.5% and watered every day. Treatment with an ecoenzyme concentration of 1.5% and daily watering exerts the best influence on the growth of red chili plants. Keywords: Liquid fertilizer, leaf area and image-J