Ibrahim Abubakar
Department of Biology-Chemistry, Idris Koko Technical College, Farfaru, Sokoto, Nigeria

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Application of Bacterial Biofilm in Remediation of Crude Oil Polluted Mangroove Forest Water Abdulganiyu Mohammed Galadima; Reuben Nwoye Okechi; Angela Chika Udebuani; Ibrahim Abubakar; Said Sani Said
Journal of Bioresources and Environmental Sciences Vol 5, No 1 (2026): April 2026
Publisher : BIORE Scientia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61435/jbes.2025.19983

Abstract

Oil and gas industrial activities have caused serious water pollution and adverse health effects on human, animals, plants, and aquatic organisms. Biofilms have significant potential for remediation of polluted water. This study aimed at evaluating the efficiency of bacterial biofilm in bioremediation of crude oil polluted mangrove forest water of Gbaramatu Kingdom of Warri South-West, Delta state, Nigeria. The total petroleum hydrocarbon (TPH) was determined by gravimetric while total hydrocarbon content (THC) and total oil and grease (TOG) were estimated using Fourier Transform Infrared (FTIR) spectroscopic technique. The screening of bacterial isolates for biofilm formation was conducted using microtitre plate assay and tube method. The crude oil degradation capacities of the bacterial isolates and bacterial biofilms were determined by gravimetric technique and GC-MS analysis. The mangrove water contains significant (p < 0.05) amount of THC (823.05 mg/L), TPH (1132.11 mg/L), and TOG (333.99 mg/L). The result showed that Pseudomonas isolates exhibited high significant (p < 0.05) growth intensity (80.94 %) compared to the Bacillus (75.04 %) and Micrococcus (53.20 %) isolates. The Pseudomonas (1.070 OD) and Micrococcus (0.818 OD) produced maximum significant (p < 0.05) amount of biofilm compared to the Bacillus (0.082 OD). The bacterial biofilms displayed more significant (p < 0.05) crude oil degradation efficiency compared to the mixed bacterial isolates. More number of peaks, each represents different petroleum components were observed in the untreated water sample. The water sample treated with the bacterial biofilm showed disappearance of these peaks with few peaks of low intensity. Total degradation of short-chain hydrocarbons and low molecular weight PAHs coupled with partial degradation of long-chain hydrocarbons and high molecular weight PAHs by the biofilm was observed. The bacterial biofilms are highly effective in degradation of crude oil components than the bacterial isolates indicating that the biofilms are significant agents for bioremediation of crude oil polluted environment.
Application of Activated Carbon Derived from Typha domingensis in Industrial Wastewater Treatment Jabir Danyaya Aliyu; Ibrahim Abubakar; Zayyanu Abdullahi; Sadiq Musa
Journal of Bioresources and Environmental Sciences Accepted Issue
Publisher : BIORE Scientia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61435/jbes.2026.20003

Abstract

Water pollution due to discharge of industrial wastes into aquatic environment remains a major challenge associated with adverse health effects and environmental destruction worldwide. The activated carbon was produced from the plant biosorbent using potassium hydroxide activator method. The activated carbon produced was characterized by surface characteristics and Fourier Transform Infrared (FTIR) spectroscopic technique. The levels of heavy metals in the treated and untreated industrial wastewater sample were determined by atomic absorption spectroscopic (AAS) technique. Batch adsorption study was conducted using the American Public Health Association (APHA) method. The mechanism of heavy metals adsorption capacity of the plant-derived activated carbon was evaluated using Freundlich isotherm and Langmuir isotherm models. The result showed that the plant-derived activated carbon derived exhibited significant (p < 0.05) value of surface area (570.33 m2/g), pore volume (2.56 g/cm3), pH (6.46), conductivity (64.03 µS/cm), moisture content (20.86 %), and ash content (7.53 %) coupled with low value of porosity (0.82 %), bulk density (0.53 g/cm3), apparent density (0.32 g/cm3), and real density (1.91 g/cm3).. The FT-IR spectrum of the activated carbon displayed various band peaks at wavenumber ranged 3350 – 801 cm−1 indicating stretching of C–H, C=O, C–C C−O, and O−H. The untreated wastewater sample demonstrated high significant (p < 0.05) amount of cadmium (0.23 mg/L), cobalt (0.83 mg/L), lead (3.02 mg/L), manganese (1.47 mg/L), nickel (0.62 mg/L), and chromium (0.60 mg/L). The plant-derived activated carbon exhibited high significant (p < 0.05) percentage efficiency for removal of cadmium (95.55 %), cobalt (96.48 %), lead (97.16 %), manganese (99.25 %), nickel (96.09 %), and chromium (96.88 %) from the industrial wastewater sample. The results of this study showed that the regression coefficient (R²) values of cadmium, cobalt, lead, manganese, nickel, and chromium for the Langmuir isotherm are higher than that demonstrated by the metals for the Freundlich isotherm model. The experimental equilibrium data for cadmium, cobalt, lead, manganese, nickel, and chromium were best fitted to the Langmuir isotherm model than the Freundlich isotherm model. The activated carbon derived from the roots of Typha domingensis demonstrated high adsorption capacity for removal of cadmium, cobalt, lead, manganese, nickel, and chromium from the industrial wastewater.