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All Journal Bioscientist : Jurnal Ilmiah Biologi
Rizki, Firnanda Ayu
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences

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Analisis In Silico Filogenetik Coffea sp. Berdasarkan Gen matK dan rbcL: Dasar Pemuliaan Profil Sensorik Rizki, Firnanda Ayu; Isnawati
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18204

Abstract

This study aimed to analyze phylogenetic relationships, genetic distances, and the potential for successful hybridization based on concatenated matK and rbcL sequences. The study was conducted in silico on 11 coffee species obtained from the NCBI GenBank database. Sequence data were analyzed using bioinformatics tools for alignment, concatenated sequences, phylogenetic reconstruction, and genetic distance calculation. Phylogenetics produced a stable and accurate topology. Species showed three categories of hybridization compatibility based on genetic distance values; high ensured gene introgression stability, moderate produced heterotic hybrids, and low risked incompatibility and genetic barriers. The study concluded that species with low genetic distance and close kinship have the potential for successful hybridization. The integration of phylogenetics and genetic distance based on matK and rbcL sequences proved effective in mapping interspecies compatibility oriented towards sensory profile development. These findings provide a strategic foundation for the efficiency of modern coffee breeding programs with superior taste and aroma characteristics.
Co-Authors ISNAWATI